Chu Won-Shik, Park Hyeongryool, Moon Sangjun
Department of Mechanical Convergence Engineering, Gyeongsang National University, Changwon 51391, Gyeongsangnam-do, Republic of Korea.
Cyberneticsimagingsystems Co., Ltd., Changwon 51391, Gyeongsangnam-do, Republic of Korea.
Bioengineering (Basel). 2023 Sep 18;10(9):1092. doi: 10.3390/bioengineering10091092.
This study focuses on developing and characterizing a novel 3-dimensional cell-laden micro-patterned porous structure from a mechanical engineering perspective. Tissue engineering holds great promise for repairing damaged organs but faces challenges related to cell viability, biocompatibility, and mechanical strength. This research aims to overcome these limitations by utilizing gelatin methacrylate hydrogel as a scaffold material and employing a photolithography technique for precise patterned fabrication. The mechanical properties of the structure are of particular interest in this study. We evaluate its ability to withstand external forces through compression tests, which provide insights into its strength and stability. Additionally, structural integrity is assessed over time to determine its performance in in vitro and potential in vivo environments. We investigate cell viability and proliferation within the micro-patterned porous structure to evaluate the biological aspects. MTT assays and immunofluorescence staining are employed to analyze the metabolic activity and distribution pattern of cells, respectively. These assessments help us understand the effectiveness of the structure in supporting cell growth and tissue regeneration. The findings of this research contribute to the field of tissue engineering and provide valuable insights for mechanical engineers working on developing scaffolds and structures for regenerative medicine. By addressing challenges related to cell viability, biocompatibility, and mechanical strength, we move closer to realizing clinically viable tissue engineering solutions. The novel micro-patterned porous structure holds promise for applications in artificial organ development and lays the foundation for future advancements in large soft tissue construction.
本研究从机械工程的角度出发，致力于开发和表征一种新型的载有细胞的三维微图案化多孔结构。组织工程在修复受损器官方面前景广阔，但面临着与细胞活力、生物相容性和机械强度相关的挑战。本研究旨在通过利用甲基丙烯酸明胶水凝胶作为支架材料，并采用光刻技术进行精确的图案化制造来克服这些限制。该结构的机械性能是本研究特别关注的重点。我们通过压缩试验评估其承受外力的能力，这有助于深入了解其强度和稳定性。此外，还会随时间评估结构完整性，以确定其在体外环境中的性能以及在体内环境中的潜力。我们研究微图案化多孔结构内的细胞活力和增殖情况，以评估其生物学特性。分别采用MTT法和免疫荧光染色法分析细胞的代谢活性和分布模式。这些评估有助于我们了解该结构在支持细胞生长和组织再生方面的有效性。本研究结果为组织工程领域做出了贡献，并为致力于开发再生医学支架和结构的机械工程师提供了有价值的见解。通过应对与细胞活力、生物相容性和机械强度相关的挑战，我们朝着实现临床上可行的组织工程解决方案又迈进了一步。这种新型的微图案化多孔结构在人工器官开发中具有应用前景，并为大型软组织构建的未来进展奠定了基础。
