Mao Jing, Zhao Yunjiao, Wang Lechen, Wu Tao, Jin Yan, Meng Jing, Zhang Min
State Key Laboratory of Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science & Technology, Tianjin 300457, China.
School of Biological Science and Food Engineering, Chuzhou University, Chuzhou 239000, China.
Foods. 2023 Sep 15;12(18):3434. doi: 10.3390/foods12183434.
Sea cucumber peptides have been proven to exhibit a variety of biological activities. Ulcerative colitis (UC) is a chronic disease characterized by diffuse inflammation of the mucosa of the rectum and colon with increasing incidence and long duration, and is difficult to cure. The effect of sea cucumber peptide on UC is currently unknown. In this study, 1.5% dextran sulfate sodium (DSS) was added to the drinking water of mice to induce a UC model, and the daily doses of sea cucumber peptide (SP) solution of 200 mg/kg·BW, 500 mg/kg·BW, and 1000 mg/kg·BW were given to UC mice to detect the relieving effect of SP. The results showed that SP can reduce the disease activity index (DAI) of UC mice induced by DSS and can alleviate colon shortening, intestinal tissue damage, and the loss of intestinal tight junction proteins (Claudin-1, Occludin). SP decreased the spleen index, pro-inflammatory factors (IL-1β, IL-6, TNF-α), and myeloperoxidase (MPO) levels in UC mice. SP can alleviate the imbalance of gut microbiota in UC mice, increase the abundance of the , , and , and reduce the abundance of and the , as well as alleviating the decrease in short-chain fatty acid (SCFA) content in the feces of UC mice. Notably, SP inhibited miR-155 expression in the colon tissue of UC mice and increased its target protein, suppressor of cytokine signaling 1 (SOCS1), which acts as an inflammatory inhibitor. In summary, the ameliorative effect of SP on UC may be achieved by improving the imbalance of gut microbiota and regulating the miR-155/SOCS1 axis. This study provides a new idea for developing SP as a nutritional supplement to maintain intestinal health.
海参肽已被证明具有多种生物活性。溃疡性结肠炎（UC）是一种慢性病，其特征为直肠和结肠黏膜的弥漫性炎症，发病率不断上升且病程较长，难以治愈。目前尚不清楚海参肽对UC的影响。在本研究中，向小鼠饮用水中添加1.5%的葡聚糖硫酸钠（DSS）以诱导UC模型，并给UC小鼠每日灌胃200 mg/kg·BW、500 mg/kg·BW和1000 mg/kg·BW的海参肽（SP）溶液，以检测SP的缓解效果。结果表明，SP可降低DSS诱导的UC小鼠的疾病活动指数（DAI），并可减轻结肠缩短、肠道组织损伤以及肠道紧密连接蛋白（Claudin-1、Occludin）的丢失。SP降低了UC小鼠的脾脏指数、促炎因子（IL-1β、IL-6、TNF-α）和髓过氧化物酶（MPO）水平。SP可缓解UC小鼠肠道微生物群的失衡，增加 、 和 的丰度，并降低 和 的丰度，同时缓解UC小鼠粪便中短链脂肪酸（SCFA）含量的降低。值得注意的是，SP抑制了UC小鼠结肠组织中miR-155的表达，并增加了其靶蛋白细胞因子信号传导抑制因子1（SOCS1），SOCS1作为一种炎症抑制剂发挥作用。综上所述，SP对UC的改善作用可能是通过改善肠道微生物群的失衡并调节miR-155/SOCS1轴来实现的。本研究为将SP开发为维持肠道健康的营养补充剂提供了新思路。
