College of Food Science, Southwest University, Beibei, Chongqing 400715, People's Republic of China.
Key Laboratory of Tea Science of the Ministry of Education, Hunan Agricultural University, Furong District, Changsha, Hunan 410128, People's Republic of China.
J Agric Food Chem. 2021 Sep 15;69(36):10592-10605. doi: 10.1021/acs.jafc.1c04032. Epub 2021 Aug 30.
Ripened pu-erh tea has the biological activity of antioxidation and anti-inflammation, which inhibits the related parameters of colitis. However, the role of storage-induced changes in bioactive ingredients of ripened pu-erh tea in colitis remains unclear. In this study, 3.5% dextran sulfate sodium-induced colitis mice were treated with 10 mg/kg bw/day extracts, aged 14 years (P2006) and unaged (P2020) ripened pu-erh tea, respectively, for 1 week. We found that ripened pu-erh tea, especially P2006, inhibited the intestinal oxidative stress-mediated inflammation pathway (TLR4/MyD88/ROS/p38MAPK/NF-κB p65), upregulated the expression of intestinal tight junction proteins (Mucin-2, ZO-1, occludin), promoted M2 polarization of macrophages, and in turn, improved the intestinal immune barrier, which stemmed from the reshaping of intestinal microbiota (e.g., increased and levels). Our results speculate that drinking aged ripe pu-erh tea (10 mg/kg bw/day in mice, a human equivalent dose of 7 g/60 kg bw/day) has a practical effect on alleviating and preventing the development of intestinal inflammation.
熟普茶具有抗氧化和抗炎的生物活性，可抑制结肠炎的相关参数。然而，贮藏诱导的熟普茶生物活性成分变化在结肠炎中的作用尚不清楚。在这项研究中，用 3.5%葡聚糖硫酸钠诱导结肠炎小鼠，分别用 10mg/kg bw/day 的 P2006 年和 P2020 年的未陈化熟普茶提取物处理 1 周。我们发现，熟普茶，特别是 P2006，抑制了肠道氧化应激介导的炎症途径（TLR4/MyD88/ROS/p38MAPK/NF-κB p65），上调了肠道紧密连接蛋白（粘蛋白-2、ZO-1、occludin）的表达，促进了巨噬细胞的 M2 极化，从而改善了肠道免疫屏障，这源于肠道微生物群的重塑（例如，增加 和 的水平）。我们的结果推测，饮用陈化熟普茶（小鼠中 10mg/kg bw/day，相当于 7g/60kg bw/day 的人体等效剂量）对缓解和预防肠道炎症的发展具有实际效果。
