Genome-Wide Identification of Gene Family and Functional Analysis of of Winter Rapeseed ( L.) under Cold Stress.

The largest gene families in plants were found to be Glutathione transferases (GSTs), which played significant roles in regulating plant growth, development, and stress response. Within the gene family, members were found to play a crucial role in the low-temperature response process of plants. A comprehensive study identified a total of 70 genes. Cluster analysis results demonstrated that the in () could be categorized into eight sub-families and were unevenly distributed across ten chromosomes. The 39 genes were found to be organized into 15 tandem gene clusters, with the promoters containing multiple -elements associated with low-temperature response. Cold stress was observed to stimulate the expression of 15 genes, with the gene exhibiting the highest level of expression, suggesting its significant involvement in winter 's response to low-temperature stress. Subcellular localization analysis of the BraGSTF2 protein indicated its potential expression in both the cell membrane and nucleus. The analysis of stress resistance in transgenic lines demonstrated that the over-expression of this gene resulted in significantly elevated levels of SOD, POD activity, and SP content compared to the wild type following exposure to low temperatures. These levels reached their peak after 24 h of treatment. Conversely, the MDA content was lower in the transgenic plants compared to the wild-type (WT) ( L.). Additionally, the survival rate of transgenic was higher than that of the WT , suggesting that the gene may play a crucial role in enhancing the cold stress tolerance of winter . This study lays a foundation for further research on the role of the gene in the molecular regulation of cold resistance in winter .