State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, 730070, China.
College of Agronomy, Gansu Agricultural University, Lanzhou, 730070, China.
BMC Plant Biol. 2023 Dec 7;23(1):621. doi: 10.1186/s12870-023-04639-4.
The RNA recognition motif (RRM) is primarily engaged in the processing of mRNA and rRNA following gene transcription as well as the regulation of RNA transport; it is critical in preserving RNA stability.
In this study, we identified 102 members of the RRM1 gene family in Brassica rapa, which were dispersed across 10 chromosomes with the ninth chromosome being the most extensively distributed. The RRM1 gene family members of Brassica rapa and Arabidopsis thaliana were grouped into 14 subclades (I-XIV) using phylogenetic analysis. Moreover, the results of transcriptome analysis and RT-qPCR indicated that the expression of Brapa05T000840 was upregulated in the cultivars 'Longyou 7' and 'Longyou 99' following exposure to cold stress at a temperature of 4 °C for 24 h. The levels of expression in the leaves and growth cones of the 'Longyou 7' variety were found to be significantly higher than those observed in the 'Longyou 99' variety under conditions of low temperature and NaCl stress. It illustrates the involvement of the RRM1 gene in the physiological response to both low temperature and salt stress. In addition, it was observed that the survival rate of transgenic BrRBP (Brapa05T000840) Arabidopsis thaliana plants was notably higher compared to that of wild-type plants when subjected to varying durations of low temperature treatment. Furthermore, the expression of the BrRBP gene in transgenic plants exhibited an upward trend as the duration of low temperature treatment increased, reaching its peak at 24 h. The in-vivo enzymatic activity of reactive oxygen species-scavenging enzymes were found to be significantly elevated in comparison to wild-type plants, suggesting that the BrRBP gene may enhance the cold tolerance of Arabidopsis thaliana.
This study offers a significant foundation for comprehending the regulation mechanism of the RRM1 gene family in winter Brassica rapa subjected to cold stress, as well as for finding key genes associated with cold resistance.
RNA 识别基序（RRM）主要参与基因转录后的 mRNA 和 rRNA 加工以及 RNA 运输调控；它对于 RNA 稳定性的维持至关重要。
本研究在白菜中鉴定了 102 个 RRM1 基因家族成员，它们分布在 10 条染色体上，其中第 9 条染色体分布最广泛。白菜和拟南芥的 RRM1 基因家族成员通过系统发育分析被分为 14 个亚科（I-XIV）。此外，转录组分析和 RT-qPCR 的结果表明，在 4°C 冷胁迫 24 小时后，品种‘龙游 7’和‘龙游 99’中 Brapa05T000840 的表达上调。在低温和 NaCl 胁迫下，‘龙游 7’品种叶片和生长锥中的表达水平明显高于‘龙游 99’品种。这表明 RRM1 基因参与了低温和盐胁迫的生理响应。此外，观察到在低温处理下，转 BrRBP（Brapa05T000840）拟南芥植物的存活率明显高于野生型植物。此外，随着低温处理时间的延长，转基因植物中 BrRBP 基因的表达呈上升趋势，在 24 小时达到峰值。与野生型植物相比，转基因植物中活性氧清除酶的体内酶活性显著升高，表明 BrRBP 基因可能增强了拟南芥的耐寒性。
本研究为理解冬季白菜在冷胁迫下 RRM1 基因家族的调控机制以及寻找与抗寒性相关的关键基因提供了重要基础。
