Division of Biotechnology, CSIR-Institute of Himalayan Bioresource Technology, Palampur 176061, India.
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.
Genes (Basel). 2023 Aug 29;14(9):1725. doi: 10.3390/genes14091725.
Bamboos are perennial, arborescent, monocarpic and industrially important non-timber plants. They are important for various purposes, such as carbon sequestration, biodiversity support, construction, and food and fiber production. However, traditional vegetative propagation is insufficient for bamboo multiplication. Moreover, little is known about the mechanism of gold nanoparticles (AuNPs) in vitro proliferation and regulation of physiological and biochemical properties. In this study, we investigated the impacts of citrate and cetyltrimethylammonium bromide (CTAB) coated AuNPs on in vitro proliferation, photosynthetic pigment content and antioxidant potential of (Schult. and Schult. F.) Backer ex K. Heyne. Various morpho-physiological and biochemical parameters were differentially affected along the citrate- and CTAB-coated AuNPs concentration gradients (200-600 µM). In vitro shoot proliferation, photosynthetic pigment content and antioxidant activities were higher in grown on Murashige and Skoog medium supplemented with 2 mg·L benzyladenine and 400 µM citrate-coated AuNPs than in those grown on Murashige and Skoog medium supplemented with 600 µM CTAB- coated AuNPs. Identification of genes regulating in vitro proliferation will help understand the molecular regulation of AuNPs-mediated elicitation for modulating various physiological and biochemical activities during micropropagation. Gene Ontology enrichment analysis and Kyoto Encyclopedia of Genes and Genomes pathway analyses identified differentially expressed genes associated with in vitro modulation of AuNPs-regulated biological processes and molecular functions. The findings of this study provide new insight into AuNPs-mediated elicitation of in vitro mass scale bamboo propagation.
竹子是多年生、木本、单性结实且具有重要工业用途的非木材植物。它们在碳封存、生物多样性支持、建筑以及食品和纤维生产等方面具有重要作用。然而，传统的营养繁殖方式不足以满足竹子的繁殖需求。此外，人们对金纳米粒子（AuNPs）在体外增殖的机制以及对生理生化特性的调控机制知之甚少。在本研究中，我们研究了柠檬酸和十六烷基三甲基溴化铵（CTAB）包覆的 AuNPs 对（Schult. and Schult. F.）Backer ex K. Heyne 的体外增殖、光合色素含量和抗氧化潜力的影响。在柠檬酸和 CTAB 包覆的 AuNPs 浓度梯度（200-600 µM）下，各种形态生理和生化参数均表现出不同程度的差异。在补充有 2 mg·L 苯并腺嘌呤和 400 µM 柠檬酸包覆的 AuNPs 的 Murashige 和 Skoog 培养基上生长的，其体外芽增殖、光合色素含量和抗氧化活性均高于在补充有 600 µM CTAB 包覆的 AuNPs 的 Murashige 和 Skoog 培养基上生长的。鉴定调控体外增殖的基因将有助于理解 AuNPs 介导的激发在微繁殖过程中调节各种生理生化活性的分子调控机制。基因本体论富集分析和京都基因与基因组百科全书途径分析鉴定了与 AuNPs 调控的生物过程和分子功能的体外调节相关的差异表达基因。本研究的结果为 AuNPs 介导的体外大规模竹子繁殖提供了新的见解。
