Osmolovskiy Alexander A, Kreyer Valeriana G
Faculty of Biology, Lomonosov Moscow State University, Moscow 119991, Russia.
Int J Mol Sci. 2023 Sep 8;24(18):13870. doi: 10.3390/ijms241813870.
The effect of proteinase on the proteins of the human hemostasis system, fibrin, fibrinogen, plasminogen, protein C, and factor X, was studied. These proteins are key targets for proteolytic enzymes in therapy and diagnosis of thromboembolic complications. It was shown that proteinase efficiently cleaves fibrin and fibrinogen, but does not act precisely, since it cuts all three subunits of these proteins. The proteinase did not have an activating effect on the plasminogen, a precursor of plasminogen and plasmin. The proteinase of was shown to be the first fungal proteinase with proven activating activity towards the human hemostasis system factors protein C and factor X. For protein C activation, proteinase requires Ca ions. The enzyme was found to be sensitive to thrombin inhibitors, but not to plasmin inhibitors. A proteolytic action profile of the scope of this proteinase as a proteinase with activating protein C, factor X, and plasmin-like activity was proposed.
研究了蛋白酶对人止血系统蛋白质（纤维蛋白、纤维蛋白原、纤溶酶原、蛋白C和因子X）的影响。这些蛋白质是血栓栓塞并发症治疗和诊断中蛋白水解酶的关键作用靶点。结果表明，蛋白酶能有效切割纤维蛋白和纤维蛋白原，但作用并不精准，因为它会切割这些蛋白质的所有三个亚基。该蛋白酶对纤溶酶原（纤溶酶原和纤溶酶的前体）没有激活作用。已证明该蛋白酶是第一种对人止血系统因子蛋白C和因子X具有已证实激活活性的真菌蛋白酶。对于蛋白C的激活，该蛋白酶需要钙离子。发现该酶对凝血酶抑制剂敏感，但对纤溶酶抑制剂不敏感。提出了这种具有激活蛋白C、因子X和类纤溶酶活性的蛋白酶的蛋白水解作用谱。
