Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, National Engineering Research Center for Floriculture, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, China.
Beijing Laboratory of Urban and Rural Ecological Environment, Engineering Research Center of Landscape Environment of Ministry of Education, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, China.
Int J Mol Sci. 2023 Sep 11;24(18):13950. doi: 10.3390/ijms241813950.
The gene family plays a crucial role in the cleavage of carotenoids, converting them into apocarotenoids. This process not only impacts the physiology and development of plants but also enhances their tolerance toward different stresses. However, the character of the gene family and its role in ornamental woody remain unclear. Here, ten non-redundant genes were identified from the genome, and their physicochemical characteristics were predicted. According to the phylogenetic tree, PmCCD proteins were classified into six subfamilies: CCD1, CCD4, CCD7, CCD8, NCED and CCD-like. The same subfamily possessed similar gene structural patterns and numbers of conserved motifs. Ten genes were concentrated on three chromosomes. genes exhibited interspecific collinearity with and . Additionally, genes had obvious specificity in different tissues and varieties. Compared with white-flowered 'ZLE', and genes were low-expressed in 'HJH' with yellow petals, which suggested and might be related to the formation of yellow flowers in . Nine genes could respond to NaCl or PEG treatments. These genes might play a crucial role in salt and drought resistance in . Moreover, PmVAR3 and PmSAT3/5 interacted with PmCCD4 protein in yeast and tobacco leaf cells. This study laid a foundation for exploring the role of the gene family in flower coloration and stress response in .
基因家族在类胡萝卜素的裂解中起着至关重要的作用，将其转化为脱辅基类胡萝卜素。这一过程不仅影响植物的生理和发育，还增强了它们对不同胁迫的耐受性。然而，基因家族的特征及其在观赏木本植物中的作用尚不清楚。在这里，从基因组中鉴定了十个非冗余的基因，并预测了它们的理化特性。根据系统发育树，PmCCD 蛋白被分为六个亚家族：CCD1、CCD4、CCD7、CCD8、NCED 和 CCD-like。同一亚家族具有相似的基因结构模式和数量的保守基序。十个基因集中在三条染色体上。基因在种间与 和 具有共线性。此外，基因在不同组织和品种中表现出明显的特异性。与白色花朵“ZLE”相比，黄色花瓣的“HJH”中 和 基因的表达水平较低，这表明 和 可能与 的黄花形成有关。九个基因可以响应 NaCl 或 PEG 处理。这些基因可能在盐和干旱胁迫中在中发挥关键作用。此外，PmVAR3 和 PmSAT3/5 在酵母和烟草叶片细胞中与 PmCCD4 蛋白相互作用。这项研究为探索基因家族在花色形成和胁迫反应中的作用奠定了基础。
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