State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, China.
State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, China.
Int J Biol Macromol. 2021 Jul 1;182:298-310. doi: 10.1016/j.ijbiomac.2021.03.154. Epub 2021 Mar 31.
9-cis-epoxy carotenoid dioxygenase (NCED) is a fundamental enzyme, which plays an essential role in the process of organ development and stress resistance by regulating abscisic acid (ABA) synthesis in plant. In this study, a total of 7, 7, 14 and 14 NCED genes were identified from the genomes of G. arboreum, G. raimondii, G. barbadense and G. hirsutum, respectively. Phylogenetic tree showed that all forty-two NCED genes could be classified into three groups in cotton genus. Collinear analysis revealed that the NCED genes in G. hirsutum were not amplified by tandem repeats after polyploidy events. The function of NCED genes was evaluated between two accessions with contrasting plant height. The results showed that expression of the NCED genes in dwarf accession was higher than that in taller ones. GhNCED1-silenced cotton plants confirmed that suppression of NCED genes could increase the plant height, but reduce the resistance abilities to drought and salt stress. Our study systematically identified the homologs of NCED genes and their functions in cotton, which could provide new genetic resources for improving plant height and stress in future cotton breeding.
9-顺式-环氧类胡萝卜素双加氧酶（NCED）是一种基础酶，通过调节植物中脱落酸（ABA）的合成，在器官发育和抗逆性过程中发挥着重要作用。本研究分别从山黧豆、雷蒙德氏棉、长果种棉和陆地棉基因组中鉴定出了 7、7、14 和 14 个 NCED 基因。系统发育树表明，棉属的 42 个 NCED 基因可分为 3 组。共线性分析表明，陆地棉的 NCED 基因在多倍体事件后没有通过串联重复扩增。对两个株高差异较大的品种进行 NCED 基因功能评估，结果表明矮秆品种中 NCED 基因的表达高于高秆品种。GhNCED1 沉默的棉花植株证实，抑制 NCED 基因可以增加株高，但降低对干旱和盐胁迫的抗性。本研究系统地鉴定了 NCED 基因在棉花中的同源物及其功能，为未来棉花育种中提高株高和抗逆性提供了新的遗传资源。
