广泛使用的源自 Bacmid pSM3fr 的重组鼠巨细胞病毒 MCMV-m157luc 的重新分析证实了其杂种性质。

Re-Analysis of the Widely Used Recombinant Murine Cytomegalovirus MCMV-m157luc Derived from the Bacmid pSM3fr Confirms Its Hybrid Nature.

机构信息

Institute for Clinical and Molecular Virology, Friedrich-Alexander-Universität Erlangen-Nürnberg, 91054 Erlangen, Germany.

出版信息

Int J Mol Sci. 2023 Sep 14;24(18):14102. doi: 10.3390/ijms241814102.

DOI:10.3390/ijms241814102

PMID:37762404

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10531225/

Abstract

Murine cytomegalovirus (MCMV), and, in particular, recombinant virus derived from MCMV-bacmid pSM3fr, is widely used as the small animal infection model for human cytomegalovirus (HCMV). We sequenced the complete genomes of MCMV strains and recombinants for quality control. However, we noticed deviances from the deposited reference sequences of MCMV-bacmid pSM3fr. This prompted us to re-analyze pSM3fr and reannotate the reference sequence, as well as that for the commonly used MCMV-m157luc reporter virus. A correct reference sequence for this frequently used pSM3fr, containing a repaired version of m129 (MCK-2) and the luciferase gene instead of ORF m157, was constructed. The new reference also contains the original bacmid sequence, and it has a hybrid origin from MCMV strains Smith and K181.

摘要

鼠巨细胞病毒（MCMV），特别是源自 MCMV-bacmid pSM3fr 的重组病毒，被广泛用作人类巨细胞病毒（HCMV）的小动物感染模型。我们对 MCMV 株和重组病毒的完整基因组进行了测序，以进行质量控制。然而，我们注意到与 MCMV-bacmid pSM3fr 的已提交参考序列存在差异。这促使我们重新分析 pSM3fr 并重新注释参考序列，以及常用的 MCMV-m157luc 报告病毒。构建了一个经常使用的 pSM3fr 的正确参考序列，其中包含 m129（MCK-2）的修复版本和荧光素酶基因，而不是 ORF m157。新的参考序列还包含原始 bacmid 序列，并且具有来自 MCMV 株 Smith 和 K181 的混合起源。