College of Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524091, China.
Noble Research Institute LLC, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA.
Int J Mol Sci. 2023 Jan 27;24(3):2484. doi: 10.3390/ijms24032484.
The MYB gene family widely exists in the plant kingdom and participates in the regulation of plant development and stress response. Pearl millet ( R. Br.), as one of the most important cereals, is not only considered a good source of protein and nutrients but also has excellent tolerances to various abiotic stresses (e.g., salinity, water deficit, etc.). Although the genome sequence of pearl millet was recently published, bioinformatics and expression pattern analysis of the MYB gene family are limited. Here, we identified 208 PgMYB genes in the pearl millet genome and employed 193 high-confidence candidates for downstream analysis. Phylogenetic and structural analysis classified these PgMYBs into four subgroups. Eighteen pairs of segmental duplications of the PgMYB gene were found using synteny analysis. Collinear analysis revealed pearl millet had the closest evolutionary relationship with foxtail millet. Nucleotide substitution analysis (Ka/Ks) revealed PgMYB genes were under purifying positive selection pressure. Reverse transcription-quantitative PCR analysis of eleven R2R3-type PgMYB genes revealed they were preferentially expressed in shoots and seeds and actively responded to various environment stimuli. Current results provide insightful information regarding the molecular features of the MYB family in pearl millet to support further functional characterizations.
MYB 基因家族广泛存在于植物界，参与植物发育和应激反应的调控。珍珠粟（R. Br.）作为最重要的谷物之一，不仅被认为是蛋白质和营养物质的良好来源，而且对各种非生物胁迫（如盐度、水分亏缺等）具有优异的耐受性。尽管珍珠粟的基因组序列最近已经公布，但该基因家族的生物信息学和表达模式分析还很有限。在这里，我们在珍珠粟基因组中鉴定了 208 个 PgMYB 基因，并对 193 个高可信度的候选基因进行了下游分析。系统发育和结构分析将这些 PgMYBs 分为四个亚组。通过共线性分析发现，18 对 PgMYB 基因发生了片段复制。共线性分析表明，珍珠粟与谷子的进化关系最密切。核苷酸取代分析（Ka/Ks）表明，PgMYB 基因受到正向选择压力的影响。对 11 个 R2R3 型 PgMYB 基因的反转录定量 PCR 分析表明，它们在茎和种子中优先表达，并对各种环境刺激做出积极响应。目前的研究结果为珍珠粟 MYB 家族的分子特征提供了深入的信息，为进一步的功能表征提供了支持。
