Industrial Crops Institute of Hubei Academy of Agricultural Sciences, Key Laboratory of Vegetable Ecological Cultivation on Highland, Ministry of Agriculture and Rural Affairs, Wuhan 430064, China.
MARA Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River, Co-Construction by Ministry and Province, College of Agriculture, Yangtze University, Jingzhou 434025, China.
Int J Mol Sci. 2024 Jan 11;25(2):929. doi: 10.3390/ijms25020929.
The domains of unknown function (DUF) superfamilies contain proteins with conserved amino acid sequences without known functions. Among them, DUF668 was indicated widely involving the stress response of plants. However, understanding ZoDUF668 is still lacking. Here, 12 genes were identified in ginger by the bioinformatics method and unevenly distributed on six chromosomes. Conserved domain analysis showed that members of the same subfamily had similar conserved motifs and gene structures. The promoter region of contained the light, plant hormone and stress-responsive elements. The prediction of miRNA targeting relationship showed that nine ginger miRNAs targeted four genes through cleavage. The expression patterns of 12 genes under biotic and abiotic stress were analyzed using RT-qPCR. The results showed that the expression of seven genes was significantly downregulated under infection, six genes were upregulated under cold stress, and five genes were upregulated under waterlogging stress. These results indicate that the gene has different expression patterns under different stress conditions. This study provides excellent candidate genes and provides a reference for stress-resistance research in ginger.
未知功能（DUF）超家族的结构域包含具有保守氨基酸序列但无已知功能的蛋白质。其中，DUF668 广泛参与植物的应激反应。然而，对 ZoDUF668 的了解仍然不足。本研究采用生物信息学方法从生姜中鉴定出 12 个基因，这些基因不均匀分布在 6 条染色体上。保守结构域分析表明，同一亚家族的成员具有相似的保守基序和基因结构。含有光、植物激素和应激响应元件。miRNA 靶关系的预测表明，9 个生姜 miRNA 通过切割靶向 4 个 基因。采用 RT-qPCR 分析 12 个基因在生物和非生物胁迫下的表达模式。结果表明，7 个 基因在 感染下的表达显著下调，6 个 基因在冷胁迫下上调，5 个 基因在淹水胁迫下上调。这些结果表明， 基因在不同胁迫条件下有不同的表达模式。本研究为生姜的抗逆性研究提供了优异的候选基因，为生姜的抗逆性研究提供了参考。
