Senchenkov Vladislav Yu, Lyakhovchenko Nikita S, Nikishin Ilya A, Myagkov Dmitry A, Chepurina Anna A, Polivtseva Valentina N, Abashina Tatiana N, Delegan Yanina A, Nikulicheva Tatiana B, Nikulin Ivan S, Bogun Alexander G, Solomentsev Viktor I, Solyanikova Inna P
Department of Biochemistry, Medical Institute, Belgorod State National Research University, 308015 Belgorod, Russia.
Department of Biotechnology and Microbiology, Institute of Pharmacy, Chemistry and Biology, Belgorod State National Research University, 308015 Belgorod, Russia.
Microorganisms. 2023 Sep 5;11(9):2235. doi: 10.3390/microorganisms11092235.
Bacteria, designated as A1.1 and A1.2, were isolated from poultry waste based on the ability to form ammonia on LB nutrient medium. Whole genome sequencing identified the studied strains as VKM B-3700D (A1.1) and VKM B-3701D (A1.2) with genome sizes of 5462638 and 4158287 bp, respectively. In the genome of VKM B-3701D, gene clusters of secondary metabolites of bacillin, subtilisin, bacilisin, surfactin, bacilliacin, fengycin, sactipeptide, and ratipeptide (spore killing factor) with potential antimicrobial activity were identified. Clusters of coronimine and peninodin production genes were found in VKM B-3700D. Information on coronimine in bacteria is extremely limited. The study of the individual properties of the strains showed that the cultures are capable of biosynthesis of a number of enzymes, including amylases. The VKM V-3701D inhibited the growth of bacterial test cultures and reduced the growth rate of the mold fungus VKM F-1754 by 70% relative to the control. The antimicrobial activity of VKM V-3700D was insignificant. At the same time, a mixture of cultures VKM B-3700D/ VKM B-3701D reduced the growth rate of VKM F-1754 by 24.5%. It has been shown that strain A1.1 is able to use nitrogen compounds for assimilation processes. It can be assumed that VKM V-3700D belongs to the group of nitrifying or denitrifying microorganisms, which may be important in developing methods for reducing nitrogen load and eutrophication.
从家禽粪便中分离出了命名为A1.1和A1.2的细菌，分离依据是它们在LB营养培养基上形成氨的能力。全基因组测序确定所研究的菌株分别为VKM B - 3700D（A1.1）和VKM B - 3701D（A1.2），基因组大小分别为5462638和4158287碱基对。在VKM B - 3701D的基因组中，鉴定出了具有潜在抗菌活性的杆菌肽、枯草杆菌蛋白酶、芽孢杆菌素、表面活性素、芽孢杆菌酸、丰原素、硫肽和脂肽（孢子杀伤因子）等次生代谢产物的基因簇。在VKM B - 3700D中发现了冠菌素和青霉定生产基因簇。关于细菌中冠菌素的信息极为有限。对这些菌株个体特性的研究表明，这些培养物能够生物合成多种酶，包括淀粉酶。VKM V - 3701D抑制了细菌测试培养物的生长，并使霉菌VKM F - 1754的生长速率相对于对照降低了70%。VKM V - 3700D的抗菌活性不显著。同时，VKM B - 3700D/VKM B - 3701D的混合培养物使VKM F - 1754的生长速率降低了24.5%。已表明菌株A1.1能够利用氮化合物进行同化过程。可以推测VKM V - 3700D属于硝化或反硝化微生物组，这在开发减少氮负荷和富营养化的方法中可能很重要。
