Martinez-Hernandez Fernando, Sanchez-Aguillon Fabiola, Martinez-Ocaña Joel, Gonzalez-Arenas Nelly Raquel, Romero-Valdovinos Mirza, Lopez-Escamilla Eduardo, Maravilla Pablo, Villalobos Guiehdani
Departamento de Ecologia de Agentes Patogenos, Hospital General "Dr. Manuel Gea Gonzalez", Mexico City 14080, Mexico.
Laboratorio de Investigación del Departamento de Biologia Molecular e Histocompatibilidad, Hospital General "Dr. Manuel Gea Gonzalez", Mexico City 14080, Mexico.
Microorganisms. 2023 Sep 5;11(9):2240. doi: 10.3390/microorganisms11092240.
In the present study, we evaluated the genetic variability of the internal transcribed spacer (ITS) region and the pyruvate:ferredoxin oxidoreductase () A gene of from female patients and its possible implications in the host-parasite relationship. Phylogenetic and genetics of populations analyses were performed by analyzing sequences of the ITS region and partial A gene of clinical samples with , as previously documented. Alignments of protein sequences and prediction of three-dimensional structure were also performed. Although no correlation between the main clinical characteristics of the samples and the results of phylogeny was found, a median-joining analysis of ITS haplotypes showed two main clusters. Also, A, due to its phylogenetic divergence, could be used as a marker to confirm the genus and species of trichomonads. Alignment of protein sequences and prediction of three-dimensional structure showed that PFOR A had a highly conserved structure with two synonymous mutations in the PFOR domain, substituting a V for a G or a S for a P. Our results suggest that the role of genetic variability of PFOR and ITS may not be significant in the symptomatology of this pathogen; however, their utility as genus and species markers in trichomonads is promising.
在本研究中，我们评估了女性患者阴道毛滴虫内部转录间隔区（ITS）区域和丙酮酸：铁氧化还原蛋白氧化还原酶（PFOR）A基因的遗传变异性及其在宿主 - 寄生虫关系中的潜在影响。如先前所述，通过使用MEGA分析临床样本的ITS区域和部分PFOR A基因序列，进行了群体系统发育和遗传学分析。还进行了蛋白质序列比对和三维结构预测。尽管未发现样本的主要临床特征与系统发育结果之间存在相关性，但ITS单倍型的中位连接分析显示出两个主要聚类。此外，由于PFOR A在系统发育上的差异，它可作为确认滴虫属和种的标志物。蛋白质序列比对和三维结构预测表明，PFOR A具有高度保守的结构，在PFOR结构域中有两个同义突变，将一个V替换为G或将一个S替换为P。我们的结果表明，PFOR和ITS的遗传变异在该病原体的症状学中可能作用不显著；然而，它们作为滴虫属和种标志物的效用很有前景。
