Department of Oral Physiology, School of Dentistry, Pusan National University, Yangsan 50612, Republic of Korea.
Periodontal Disease Signaling Network Research Center (MRC), Pusan National University, Yangsan 50612, Republic of Korea.
Molecules. 2023 Sep 20;28(18):6717. doi: 10.3390/molecules28186717.
Hispidulin is a natural bioactive flavonoid that has been studied for its potential therapeutic properties, including its anti-inflammatory, antioxidant, and neuroprotective effects. The aim of this study was to explore whether hispidulin could inhibit the endothelial inflammation triggered by () lipopolysaccharide (LPS). The adhesion of monocytes to the vascular endothelium was evaluated through in vitro and ex vivo monocyte adhesion assays. We analyzed the migration of monocytes across the endothelial layer using a transmigration assay. The results showed that treatment with hispidulin decreased the LPS-induced adhesion of monocytes to endothelial cells and their migration by suppressing the LPS-triggered expression of intercellular adhesion molecule-1 (ICAM-1) through downregulating nuclear factor-қB (NF-қB). In addition, hispidulin inhibited LPS-induced mitogen-activated protein kinases (MAPKs) and AKT in endothelial cells. Altogether, the results indicate that hispidulin suppresses the vascular inflammation induced by LPS. Mechanistically, it prevents the adhesion of monocytes to the vascular endothelium and migration and inhibits NF-қB, MAPKs, and AKT signaling in endothelial cells.
蛇床定是一种天然生物活性黄酮类化合物，其潜在的治疗特性包括抗炎、抗氧化和神经保护作用。本研究旨在探讨蛇床定是否能抑制（）脂多糖（LPS）引发的内皮炎症。通过体外和离体单核细胞黏附试验评估单核细胞与血管内皮的黏附。我们使用迁移试验分析单核细胞穿过内皮层的迁移。结果表明，蛇床定通过下调核因子-κB（NF-κB），抑制 LPS 触发的细胞间黏附分子-1（ICAM-1）的表达，减少 LPS 诱导的单核细胞与内皮细胞的黏附和迁移。此外，蛇床定抑制了内皮细胞中 LPS 诱导的丝裂原活化蛋白激酶（MAPKs）和 AKT。总之，这些结果表明蛇床定抑制了 LPS 诱导的血管炎症。其机制可能是防止单核细胞黏附于血管内皮细胞，并抑制内皮细胞中 NF-κB、MAPKs 和 AKT 信号通路。
