Cell & Matrix Research Institute, Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu, Republic of Korea.
Immunoregulatory Materials Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup, Republic of Korea.
Biomed Pharmacother. 2021 May;137:111359. doi: 10.1016/j.biopha.2021.111359. Epub 2021 Feb 16.
Atopic dermatitis (AD) is a chronic inflammatory skin disorder that affects 10-20% of the world's population. Therefore, the discovery of drugs for the treatment of AD is important for human health. Hispidulin (HPD; also known as scutellarein 6-methyl ether or dinatin) is a natural flavone that exerts anti-inflammatory effects. In the present study, the effectiveness of HPD on AD-like skin inflammation was investigated. We used a mouse AD model through repeated exposure of mice to 2,4-dinitrochlorobenzene and house dust mite extract (Dermatophagoides farinae extract, DFE) to the ears. In addition, tumor necrosis factor-α and interferon-γ-activated keratinocytes (HaCaT cells) were used to investigate the underlying mechanism of HPD action. Oral administration of HPD alleviated AD-like skin inflammations: it reduced ear thickness; serum immunoglobulin (Ig)E, DFE-specific IgE, and IgG2a levels; and inflammatory cell infiltration. HPD reduced the expression of pro-inflammatory cytokines and chemokines through inhibition of signal transducer and activator of transcription 1 nuclear factor-κB in HaCaT cells. Taken together, these results suggest that HPD could be a potential drug candidate for the treatment of AD.
特应性皮炎（AD）是一种慢性炎症性皮肤疾病，影响全球 10-20%的人口。因此，发现治疗 AD 的药物对于人类健康非常重要。山柰酚（HPD；也称为黄芩素 6-甲基醚或地那汀）是一种天然黄酮类化合物，具有抗炎作用。在本研究中，研究了 HPD 对 AD 样皮肤炎症的有效性。我们通过反复将小鼠暴露于 2,4-二硝基氯苯和屋尘螨提取物（粉尘螨提取物，DFE）于耳朵上来建立小鼠 AD 模型。此外，还使用肿瘤坏死因子-α和干扰素-γ激活的角质形成细胞（HaCaT 细胞）来研究 HPD 作用的潜在机制。HPD 的口服给药可缓解 AD 样皮肤炎症：它可降低耳厚度；血清免疫球蛋白（Ig）E、DFE 特异性 IgE 和 IgG2a 水平；以及炎症细胞浸润。HPD 通过抑制 HaCaT 细胞中的信号转导和转录激活子 1 核因子-κB 来减少促炎细胞因子和趋化因子的表达。综上所述，这些结果表明 HPD 可能是治疗 AD 的潜在药物候选物。
