KM Convergence Research Division, Korea Institute of Oriental Medicine, Yuseong-daero 1672, Yuseong-gu, Daejeon 34054, Korea.
Korean Convergence Medicine Major KIOM, University of Science & Technology (UST), 1672 Yuseongdae-ro, Yuseong-gu, Daejeon 34054, Korea.
Nutrients. 2022 Sep 5;14(17):3667. doi: 10.3390/nu14173667.
linn has traditionally been used for the treatment of respiratory and gastrointestinal disorders in India. Although various pharmacological effects of have been studied, its effects on bone have not been clearly elucidated. Therefore, this study examined the inhibitory effect of the water extract of Linn (WEPL) on osteoclast differentiation. WEPL directly affected the osteoclast precursors and suppressed osteoclast differentiation in vitro. In addition, the expression levels of c-Fos and nuclear factor of activated T cells 1, a critical transcription factor for osteoclastogenesis, were significantly downregulated by WEPL via the suppression of the receptor activator of nuclear factor (NF)-κB ligand-induced mitogen-activated protein kinase and NF-κB signaling pathways. Consistent with the in vitro results, oral administration of WEPL (100 and 300 mpk) to ovariectomized mice for six weeks relieved the OVX-induced bone loss. We also identified phytochemicals in WEPL that are reported to exert inhibitory effects on osteoclastogenesis and/or bone loss. Collectively, the findings of our study indicate that WEPL has an anti-osteoporotic effect on OVX-induced bone loss by diminishing osteoclast differentiation, suggesting that it may be useful to treat several bone diseases caused by excessive bone resorption.
林纳通常被用于治疗印度的呼吸和胃肠道疾病。尽管已经研究了 的各种药理作用，但它对骨骼的影响尚未得到明确阐明。因此，本研究检查了 Linn 水提取物（WEPL）对破骨细胞分化的抑制作用。WEPL 直接作用于破骨细胞前体，并在体外抑制破骨细胞分化。此外，WEPL 通过抑制核因子κB 配体诱导的丝裂原活化蛋白激酶和 NF-κB 信号通路，显著下调破骨细胞生成的关键转录因子核因子活化 T 细胞 1 中的 c-Fos 的表达水平。与体外结果一致，WEPL（100 和 300 mpk）经口给予去卵巢小鼠 6 周可缓解 OVX 引起的骨丢失。我们还鉴定了 WEPL 中的植物化学物质，据报道这些物质对破骨细胞生成和/或骨丢失具有抑制作用。总之，我们的研究结果表明，WEPL 通过减少破骨细胞分化对 OVX 诱导的骨丢失具有抗骨质疏松作用，表明它可能对治疗由过度骨吸收引起的几种骨骼疾病有用。
