Liu Xiaokang, Wang Yang, Ge Wei, Cai Guangzhi, Guo Yunlong, Gong Jiyu
School of Pharmaceutical Sciences Changchun University of Chinese Medicine Changchun China.
Jilin Ginseng Academy Changchun University of Chinese Medicine Changchun China.
Food Sci Nutr. 2022 Feb 22;10(5):1592-1601. doi: 10.1002/fsn3.2782. eCollection 2022 May.
Brongn. is a medicinal and edible plant resource as well as a natural additive in the functional food market. To better understand its characteristics and efficacy, a method combining chromatographic fingerprints and antioxidant activity was proposed. A total of 21 common peaks were confirmed from liquid chromatography fingerprints and were identified as 14 flavonoids and 7 phenolic acids by ultra-high-performance liquid chromatography (UHPLC) coupled with quadrupole Orbitrap mass spectrometry (Q-Orbitrap/MS). Their antioxidant activities were evaluated by 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH), 2,2'-diazide-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), and ferric reducing antioxidant power (FRAP) assay. The results showed that all of the test samples had moderate to high antioxidant effects, with IC50 values ranging from 5.2 to 16.1 mg/ml and 1.2 to 2.8 mg/ml for DPPH and ABTS assays, and the FeSO concentrations of 1.84-4.20 mmol/L for the FRAP assay. The spectrum-effect relationship between UHPLC fingerprints and antioxidant activity was investigated through Pearson correlation analysis and Grey relational analysis (GRA) to identify the antioxidant constitutes in Brongn. The results showed that 11 compounds were greatly associated with the antioxidant activity with a correlation degree >0.80, which can be used as the quality marker of Brongn.
布隆恩是一种药食两用植物资源，也是功能食品市场中的一种天然添加剂。为了更好地了解其特性和功效，提出了一种结合色谱指纹图谱和抗氧化活性的方法。通过液相色谱指纹图谱共确认了21个共有峰，并通过超高效液相色谱（UHPLC）与四极杆轨道阱质谱（Q-Orbitrap/MS）联用鉴定为14种黄酮类化合物和7种酚酸。通过1,1-二苯基-2-三硝基苯肼（DPPH）、2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）二铵盐（ABTS）和铁还原抗氧化能力（FRAP）测定法评估了它们的抗氧化活性。结果表明，所有测试样品均具有中等至高抗氧化效果，DPPH和ABTS测定法的IC50值分别为5.2至16.1mg/ml和1.2至2.8mg/ml，FRAP测定法的FeSO浓度为1.84-4.20mmol/L。通过Pearson相关分析和灰色关联分析（GRA）研究了UHPLC指纹图谱与抗氧化活性之间的谱效关系，以鉴定布隆恩中的抗氧化成分。结果表明，11种化合物与抗氧化活性密切相关，相关度>0.80，可作为布隆恩的质量标志物。
