Matsumoto T, Matsumori H, Hosokawa Y, Abe H, Uekusa T, Kuwabara N, Fukuda Y, Ishikawa H
Acta Pathol Jpn. 1986 Aug;36(8):1163-80. doi: 10.1111/j.1440-1827.1986.tb02837.x.
To date, no reliable report on the regeneration of the intrahepatic bile duct epithelium following damage to the duct has been published. In this study, a direct instillation of paraquat dichloride into the intrahepatic bile ducts of rats was carried out, and the livers were examined under light and electron microscopy. One hour after treatment, the biliary epithelia showed degeneration and necrosis, and these changes remained for a considerable period in a large majority of the ducts examined. Three weeks after instillation, low columnar epithelium consisting of hyperchromatic nuclei and eosinophilic cytoplasm was present in a medium-sized bile duct, which was collared by marked periductal fibrosis. Electron microscopically, the eosinophilic epithelium showed a marked increase in the number of rough endoplasmic reticula, ribosomes, mitochondria, and filamentous structures, suggesting an active viability of the cell. Subsequently, the eosinophilic cells were replaced by normal-appearing epithelium, not associated with the periductal fibrosis. The data suggest that an epithelial regeneration occurred in the intrahepatic duct following injury and that this activity may be similar to that of the extrahepatic bile duct epithelium.
迄今为止，尚未发表关于肝内胆管受损后胆管上皮细胞再生的可靠报告。在本研究中，将二氯百草枯直接滴注到大鼠的肝内胆管中，并在光镜和电镜下检查肝脏。处理1小时后，胆管上皮出现变性和坏死，在大多数检查的胆管中，这些变化持续了相当长的一段时间。滴注三周后，在一条中等大小的胆管中出现了由深染细胞核和嗜酸性细胞质组成的低柱状上皮，周围有明显的导管周围纤维化。电镜下，嗜酸性上皮的粗面内质网、核糖体、线粒体和丝状结构数量显著增加，表明细胞具有活跃的活力。随后，嗜酸性细胞被外观正常的上皮取代，且与导管周围纤维化无关。数据表明，肝内胆管损伤后发生了上皮再生，且这种活动可能与肝外胆管上皮的活动相似。
