Benedetti A, Bassotti C, Rapino K, Marucci L, Jezequel A M
Clinica di Gastroenterologia, University of Ancona, School of Medicine, Italy.
J Hepatol. 1996 Mar;24(3):335-42. doi: 10.1016/s0168-8278(96)80014-6.
BACKGROUND/AIMS: Morphological and functional heterogeneity of intrahepatic bile duct epithelial cells has been suggested in situ and in isolated cholangiocytes. The aim of this study was to evaluate if: (a) bile ducts, when isolated, maintain morphometric parameters similar to ducts in situ, (b) cellular organelles show heterogeneity in ducts of different size, and (c) some features permit different classes of bile ducts to be distinguished.
Studies in situ were conducted on normal liver processed for light or electron microscopy. Data were also obtained from preparations of intrahepatic biliary tree isolated from rat liver. The whole biliary tree was cut at different levels to obtain bile ducts of different diameter. The diameter of ducts, the number of lining cells, the size and the area of individual cells, the nucleo/cytoplasmic ratio, the volume density of mitochondria, endoplasmic reticulum, Golgi complex and lysosomes have been evaluated.
The diameter of intrahepatic bile ducts ranged from 5 to 100 micrograms and the area of lining cells ranged from 8 to 100 micrograms2. A highly significant linear relationship existed between duct diameter and bile duct epithelial cell area (r = 0.97, p < 0.001) or number of lining cells (r = 0.96, p < 0.001). The volume density of mitochondria ranged from 7.58 +/- 2.0% of cytoplasmic volume in the smallest isolated bile ducts to 8.50 +/- 2.7% in the largest (p = NS). The volume density of lysosomes was low and was not significantly different in ducts of different size. Rough endoplasmic reticulum was inconspicuous in the smallest ducts and increased only slightly in the largest. The inverse relationship between the nucleo/cytoplasmic ratio and duct diameter was striking (r = -0.78, p < 0.001). All morphometric data were equivalent if bile ducts were evaluated in situ or in isolated fragments. Taken together, the data allowed bile ducts to be classified into 3 classes: < 10, 10-50, and > 50 micrograms in diameter.
Our data show that (a) isolated bile ducts maintain morphometric characteristics similar to the tissue in situ, (b) a low grade of morphological heterogeneity is evident for intracellular organelles in ducts of different diameter and (c) the diameter of ducts, the number of lining cells and especially the nucleo/cytoplasmic ratio may indicate the origin of fragments examined where functional studies are being considered.
背景/目的：肝内胆管上皮细胞的形态和功能异质性已在原位及分离的胆管细胞中得到提示。本研究的目的是评估：（a）分离后的胆管是否保持与原位胆管相似的形态学参数；（b）不同大小胆管的细胞器是否存在异质性；（c）某些特征是否有助于区分不同类型的胆管。
对经光镜或电镜处理的正常肝脏进行原位研究。数据还来自从大鼠肝脏分离的肝内胆管树标本。在不同水平切断整个胆管树以获得不同直径的胆管。评估了胆管直径、衬里细胞数量、单个细胞的大小和面积、核质比、线粒体、内质网、高尔基体和溶酶体的体积密度。
肝内胆管直径范围为5至100微米，衬里细胞面积范围为8至100平方微米。胆管直径与胆管上皮细胞面积（r = 0.97，p < 0.001）或衬里细胞数量（r = 0.96，p < 0.001）之间存在高度显著的线性关系。线粒体的体积密度范围从最小的分离胆管中占细胞质体积的7.58±2.0%到最大胆管中的8.50±2.7%（p = 无显著差异）。溶酶体的体积密度较低，在不同大小的胆管中无显著差异。在最小的胆管中粗面内质网不明显，在最大的胆管中仅略有增加。核质比与胆管直径之间的负相关关系显著（r = -0.78，p < 0.001）。如果对胆管进行原位或分离片段评估，所有形态学数据都是等效的。综合来看，这些数据可将胆管分为3类：直径<10微米、10 - 50微米和>50微米。
我们的数据表明：（a）分离的胆管保持与原位组织相似的形态学特征；（b）不同直径胆管的细胞内细胞器存在低度形态异质性；（c）胆管直径、衬里细胞数量，尤其是核质比可能表明在考虑进行功能研究时所检查片段的来源。
