Landa-Moreno Cinthia I, Trejo-Hurtado Cristian M, Lemus-de la Cruz Jenaro, Peña-Montes Donovan J, Murillo-Villicaña Marina, Huerta-Cervantes Maribel, Montoya-Pérez Rocío, Salgado-Garciglia Rafael, Manzo-Avalos Salvador, Cortés-Rojo Christian, Monribot-Villanueva Juan Luis, Guerrero-Analco José Antonio, Saavedra-Molina Alfredo
Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Francisco J. Múgica S/N, Morelia 58030, Michoacán, Mexico.
Red de Estudios Moleculares Avanzados, Clúster BioMimic, Instituto de Ecología, A.C., Xalapa 91073, Veracruz, Mexico.
Plants (Basel). 2023 Sep 7;12(18):3196. doi: 10.3390/plants12183196.
Diabetes mellitus (DM) is a metabolic disorder characterized by persistent hyperglycemia. This state may lead to an increase in oxidative stress, which contributes to the development of diabetes complications, including diabetic kidney disease. is a traditional medicinal herb in Asia, employed in the treatment of several diseases, including DM. In this study, we investigated the antioxidant effect of the ethyl acetate extract of both in vitro and on kidneys of streptozotocin-induced diabetic male rats. Firstly, phytochemicals were identified via UPLC-MS/MS, and their in vitro antioxidant capabilities were evaluated. Subsequently, male Wistar rats were assigned into four groups: normoglycemic control, diabetic control, normoglycemic treated with the extract, and diabetic treated with the extract. At the end of the treatment, fasting blood glucose (FBG) levels, creatinine, blood urea nitrogen (BUN), and uric acid were estimated. Furthermore, the kidneys were removed and utilized for the determination of mitochondrial reactive oxygen species (ROS) production, mitochondrial respiratory chain complex activities, mitochondrial lipid peroxidation, glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT) activities. The in vitro findings showed that the major phytochemicals present in the extract were phenolic compounds, which exhibited a potent antioxidant activity. Moreover, the administration of the extract reduced creatinine and BUN levels, ROS production, and lipid peroxidation and improved mitochondrial respiratory chain complex activity and GSH-Px, SODk, and CAT activities when compared to the diabetic control group. In conclusion, our data suggest that the ethyl acetate extract of possesses renoprotective effects by reducing oxidative stress on the kidneys of streptozotocin-induced diabetic male rats.
糖尿病（DM）是一种以持续性高血糖为特征的代谢紊乱疾病。这种状态可能导致氧化应激增加，进而促使糖尿病并发症的发生，包括糖尿病肾病。[植物名称]是亚洲一种传统草药，用于治疗多种疾病，包括糖尿病。在本研究中，我们研究了[植物名称]乙酸乙酯提取物在体外以及对链脲佐菌素诱导的糖尿病雄性大鼠肾脏的抗氧化作用。首先，通过超高效液相色谱-串联质谱法（UPLC-MS/MS）鉴定植物化学成分，并评估其体外抗氧化能力。随后，将雄性Wistar大鼠分为四组：正常血糖对照组、糖尿病对照组、提取物治疗的正常血糖组和提取物治疗的糖尿病组。在治疗结束时，测定空腹血糖（FBG）水平、肌酐、血尿素氮（BUN）和尿酸。此外，取出肾脏用于测定线粒体活性氧（ROS）生成、线粒体呼吸链复合物活性、线粒体脂质过氧化、谷胱甘肽过氧化物酶（GSH-Px）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性。体外研究结果表明，提取物中的主要植物化学成分是酚类化合物，它们具有强大的抗氧化活性。此外，与糖尿病对照组相比，[植物名称]提取物给药可降低肌酐和BUN水平、ROS生成和脂质过氧化，并改善线粒体呼吸链复合物活性以及GSH-Px、SOD和CAT活性。总之，我们的数据表明，[植物名称]乙酸乙酯提取物通过减轻链脲佐菌素诱导的糖尿病雄性大鼠肾脏的氧化应激而具有肾脏保护作用。
