Baumli Julia, Antal Norbert, Casoni Dorina, Cimpoiu Claudia
Faculty of Chemistry and Chemical Engineering, Babes-Bolyai University, 11 Arany Janos, 400028 Cluj-Napoca, Romania.
Research Center for Advanced Chemical Analysis, Instrumentation and Chemometrics, 11 Arany Janos, 400028 Cluj-Napoca, Romania.
Plants (Basel). 2023 Sep 11;12(18):3233. doi: 10.3390/plants12183233.
In recent years, the interest in natural remedies has increased, so it is important to analyze the plants widely distributed in nature but whose composition is little known. The main objective of the present work is to obtain information based on the profiles of secondary metabolites and antioxidant activity in , a very widespread but little studied plant, with the aim of revealing the differences compared to . First, the optimization of enzymatic extraction assisted by ultrasound was carried out by the Box-Behnken method. The optimized parameters were: concentration of the enzyme-3.3% cellulase, temperature-55 °C, and the extraction time-40.00 min. The efficiency was estimated based on the content of iridoids, the main class of secondary metabolites from Second, the secondary metabolites profiles of the nettle extracts were obtained by thin-layer chromatography using both normal and reverse phases and by RP-UHPLC. The antioxidant activity was evaluated using DPPH and ABTS radicals. The obtained results revealed significant differences between the two nettle species, both in terms of the phytochemical compounds, as well as the antioxidant activity, confirming the fact that has a high potential to be used in phytomedicine.
近年来，人们对天然药物的兴趣有所增加，因此分析自然界中广泛分布但其成分鲜为人知的植物非常重要。本研究的主要目的是基于一种分布广泛但研究较少的植物的次生代谢产物谱和抗氧化活性获取信息，旨在揭示与[另一植物]相比的差异。首先，采用Box-Behnken方法对超声辅助酶提取进行优化。优化后的参数为：酶浓度-3.3%纤维素酶、温度-55℃、提取时间-40.00分钟。基于环烯醚萜类化合物的含量来评估效率，环烯醚萜类化合物是[该植物]次生代谢产物的主要类别。其次，通过正相和反相薄层色谱以及反相超高效液相色谱法获得荨麻提取物的次生代谢产物谱。使用DPPH和ABTS自由基评估抗氧化活性。所得结果表明，这两种荨麻在植物化学化合物以及抗氧化活性方面均存在显著差异，证实了[该植物]在植物医学中具有很高的应用潜力。
