Skotadis Evangelos, Aslanidis Evangelos, Tsekenis Georgios, Panagopoulou Chryssi, Rapesi Annita, Tzourmana Georgia, Kennou Stella, Ladas Spyridon, Zeniou Angelos, Tsoukalas Dimitris
Department of Applied Physics, National Technical University of Athens, 15780 Athens, Greece.
Biomedical Research Foundation, Academy of Athens, 4 Soranou Ephessiou Street, 11527 Athens, Greece.
Sensors (Basel). 2023 Sep 12;23(18):7818. doi: 10.3390/s23187818.
A hybrid noble nanoparticle/DNAzyme electrochemical biosensor is proposed for the detection of Pb, Cd, and Cr. The sensor takes advantage of a well-studied material that is known for its selective interaction with heavy metal ions (i.e., DNAzymes), which is combined with metallic nanoparticles. The double-helix structure of DNAzymes is known to dissociate into smaller fragments in the presence of specific heavy metal ions; this results in a measurable change in device resistance due to the collapse of conductive inter-nanoparticle DNAzyme bridging. The paper discusses the effect of DNAzyme anchoring groups (i.e., thiol and amino functionalization groups) on device performance and reports on the successful detection of all three target ions in concentrations that are well below their maximum permitted levels in tap water. While the use of DNAzymes for the detection of lead in particular and, to some extent, cadmium has been studied extensively, this is one of the few reports on the successful detection of chromium (III) via a sensor incorporating DNAzymes. The sensor showed great potential for its future integration in autonomous and remote sensing systems due to its low power characteristics, simple and cost-effective fabrication, and easy automation and measurement.
一种用于检测铅、镉和铬的混合贵金属纳米颗粒/脱氧核酶电化学生物传感器被提出。该传感器利用了一种经过充分研究的材料，这种材料因其与重金属离子（即脱氧核酶）的选择性相互作用而闻名，并与金属纳米颗粒相结合。已知脱氧核酶的双螺旋结构在特定重金属离子存在下会解离成较小的片段；由于导电的纳米颗粒间脱氧核酶桥接的崩溃，这会导致器件电阻发生可测量的变化。本文讨论了脱氧核酶锚定基团（即硫醇和氨基官能团）对器件性能的影响，并报告了成功检测到所有三种目标离子，其浓度远低于自来水中的最大允许水平。虽然利用脱氧核酶检测铅，特别是在一定程度上检测镉已经得到了广泛研究，但这是少数几篇关于通过包含脱氧核酶的传感器成功检测铬（III）的报告之一。该传感器因其低功耗特性、简单且经济高效的制造工艺以及易于自动化和测量，在未来集成到自主和遥感系统中显示出巨大潜力。
