Yan Shijun, Huang Baicheng, Bai Xiaofei, Zhou Ying, Guo Linghua, Wang Tongyan, Shan Yihong, Wang Yuzhou, Tan Feifei, Tian Kegong
National Research Center for Veterinary Medicine, Luoyang, China.
Front Vet Sci. 2022 Jan 25;8:797611. doi: 10.3389/fvets.2021.797611. eCollection 2021.
In China, the re-emerging pseudorabies virus (PRV) variant has caused large-scale outbreaks of pseudorabies in swine herds with classical PRV vaccine immunization since late 2011. Here, a recombinant PRV with TK/gI/gE/11k/28k deletion was constructed based on variant HN1201 strain isolated in 2012, by the bacterial artificial chromosome infectious clones. Compared with the parental virus, the recombinant PRV rHN1201 showed a similar virus grown curve and exhibited smaller plaques. The vaccination of rHN1201 could elicit an earlier and higher level of gB antibody, and the neutralizing antibodies elicited by rHN1201 were effective against both PRV classical and variant strains. Clinically, the body temperature of the pigs immunized with rHN1201 was significantly lower than that of the classical PRV vaccine immunized pigs, and the recombinant PRV could provide effective protection against the challenge with the PRV variant. These results imply that the rHN1201 could be a promising vaccine candidate for the prevention of the current epidemic of pseudorabies in China.
在中国，自2011年末以来，重新出现的伪狂犬病病毒（PRV）变异株在已用经典PRV疫苗免疫的猪群中引发了大规模伪狂犬病疫情。在此，基于2012年分离的变异株HN1201，通过细菌人工染色体感染性克隆构建了一株缺失TK/gI/gE/11k/28k的重组PRV。与亲本病毒相比，重组PRV rHN1201呈现出相似的病毒生长曲线且蚀斑较小。rHN1201疫苗接种可引发更早且更高水平的gB抗体，并且rHN1201引发的中和抗体对PRV经典株和变异株均有效。临床上，用rHN1201免疫的猪的体温显著低于用经典PRV疫苗免疫的猪，并且该重组PRV可为抵御PRV变异株的攻击提供有效保护。这些结果表明，rHN1201可能是预防当前中国伪狂犬病流行的一种有前景的疫苗候选株。
