Tong Wu, Li Guoxin, Liang Chao, Liu Fei, Tian Qing, Cao Yanyun, Li Lin, Zheng Xuchen, Zheng Hao, Tong Guangzhi
Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, Jiangsu, China.
Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.
Antiviral Res. 2016 Jun;130:110-7. doi: 10.1016/j.antiviral.2016.03.002. Epub 2016 Mar 2.
Emerging pseudorabies virus (PRV) variant have led to pseudorabies outbreaks in Chinese pig farms. The commercially available PRV vaccine provides poor protection against the PRV variant. In this study, a gE/gI deleted PRV strain JS-2012-△gE/gI was generated from a PRV variant strain using homologous DNA recombination. Compared to the parental strain JS-2012, JS-2012-△gE/gI grew slowly and showed small plaque morphology on Vero cells. The safety and immunological efficacy of JS-2012-△gE/gI was evaluated as a vaccine candidate. JS-2012-△gE/gI was avirulent to suckling piglets, but was able to provide full protection for young piglets against challenge with both the classical virulent PRV and the emerging PRV variant. After sows were vaccinated with the gE/gI-deleted strain, their suckling offspring were resistant to an otherwise lethal challenge with the classical and the variant PRVs. Piglets inoculated with JS-2012-△gE/gI did not develop PRV-specific gE-ELISA antibodies. Thus, JS-2012-△gE/gI appears to be a promising marker vaccine candidate to control PRV variant circulating in pig farms in China.
新出现的伪狂犬病病毒（PRV）变异株已导致中国猪场发生伪狂犬病疫情。市售的PRV疫苗对PRV变异株的保护效果不佳。在本研究中，利用同源DNA重组技术从一株PRV变异株中构建了一株gE/gI基因缺失的PRV毒株JS-2012-△gE/gI。与亲本毒株JS-2012相比，JS-2012-△gE/gI在Vero细胞上生长缓慢，形成的蚀斑形态较小。对JS-2012-△gE/gI作为候选疫苗的安全性和免疫效果进行了评估。JS-2012-△gE/gI对哺乳仔猪无毒力，但能够为幼猪提供完全保护，使其免受经典强毒PRV和新出现的PRV变异株的攻击。母猪接种gE/gI基因缺失毒株后，其哺乳后代对经典和变异PRV的致死性攻击具有抵抗力。接种JS-2012-△gE/gI的仔猪未产生PRV特异性gE-ELISA抗体。因此，JS-2012-△gE/gI似乎是一种有前景的标记疫苗候选物，可用于控制中国猪场中传播的PRV变异株。
