Iheagwam Franklyn Nonso, Okeke Chiamaka Obiageli, De Campos Opeyemi Christianah, Adegboye Bose Esther, Ogunlana Olubanke Olujoke, Chinedu Shalom Nwodo
Department of Biochemistry, Covenant University, P.M.B. 1023, Ota, Ogun State, Nigeria.
Covenant University Public Health and Wellbeing Research Cluster (CUPHWERC), Covenant University, P.M.B. 1023, Ota, Ogun State, Nigeria.
Toxicol Rep. 2021 Oct 11;8:1769-1776. doi: 10.1016/j.toxrep.2021.10.005. eCollection 2021.
The present study was carried out to assess the oral safety, proinflammatory and stress response effect of aqueous leaf extract (TCA) in male Wistar rats. The acute and sub-acute oral toxicity of TCA was assessed using guidelines 423 and 407 of the Organisation for Economic Co-operation and Development (OECD), respectively. Signs of clinical toxicity, morbidity and mortality were observed. The biochemical, haematological, proinflammatory, stress response and histopathological indices were assessed. In the acute toxicity study, no sign of clinical toxicity, morbidity, and mortality was observed for TCA treatment, up to 5000 mg/kg bwt. However, in the sub-acute toxicity study, repeated daily TCA treatment significantly (0.05) altered the body weight gain, plasma alkaline phosphatase activity and albumin concentration. There were no obvious morphological and macroscopic alterations in the organs investigated. TCA appear not to elicit any proinflammatory, stress, systemic and organ toxic effect when utilised at the reported dose and time frame.
本研究旨在评估雄性Wistar大鼠中叶片水提取物（TCA）的口服安全性、促炎和应激反应效应。分别使用经济合作与发展组织（OECD）的423号和407号指南评估TCA的急性和亚急性口服毒性。观察临床毒性、发病率和死亡率的体征。评估生化、血液学、促炎、应激反应和组织病理学指标。在急性毒性研究中，高达5000 mg/kg体重的TCA处理未观察到临床毒性、发病率和死亡率的体征。然而，在亚急性毒性研究中，每日重复TCA处理显著（P<0.05）改变了体重增加、血浆碱性磷酸酶活性和白蛋白浓度。在所研究的器官中未观察到明显的形态学和宏观改变。在所报道的剂量和时间范围内使用时，TCA似乎不会引发任何促炎、应激、全身和器官毒性作用。
