Ndraha Nodali, Lin Hung-Yun, Wang Chen-Yow, Hsiao Hsin-I, Lin Han-Jia
Department of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung 202301, Taiwan.
Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung 202301, Taiwan.
Food Chem (Oxf). 2023 Sep 18;7:100183. doi: 10.1016/j.fochms.2023.100183. eCollection 2023 Dec 30.
This article presents a review of recent advancements in the utilization of NAA-based techniques for detecting foodborne pathogens in food products, focusing on studies conducted within the past five years. This review revealed that recent research efforts have primarily aimed at enhancing sensitivity and specificity by improving sample pre-treatment/preparation, DNA isolation, and readout methods. Isothermal-based amplification methods, such as LAMP, RPA, RAA, and RCA, have emerged as promising approaches, providing rapid results within one h and often demonstrating comparable or superior sensitivity to conventional or qPCR methods. However, the attention paid to specific pathogens varies, with spp., spp., , and receiving more focus than norovirus and other similar pathogens. NAA-based methods have the potential to significantly contribute to food safety and public health protection. However, further advancements are necessary to fully realize their benefits.
本文综述了基于核酸扩增技术（NAA）检测食品中食源性病原体的最新进展，重点关注过去五年内开展的研究。该综述表明，近期的研究主要致力于通过改进样品预处理/制备、DNA提取和读出方法来提高灵敏度和特异性。基于等温扩增的方法，如环介导等温扩增（LAMP）、重组酶聚合酶扩增（RPA）、重组酶辅助扩增（RAA）和滚环扩增（RCA），已成为有前景的方法，可在1小时内提供快速结果，且灵敏度通常与传统或定量聚合酶链反应（qPCR）方法相当或更高。然而，对特定病原体的关注程度有所不同，某些特定菌属比诺如病毒和其他类似病原体受到更多关注。基于NAA的方法有潜力为食品安全和公共卫生保护做出重大贡献。然而，要充分实现其益处，还需要进一步的进展。
