Tongchai Panwad, Burassakarn Ati, Langsiri Nattapong, Worasilchai Navaporn, Chindamporn Ariya
Center of Excellence for Antimicrobial Resistance and Stewardship, Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand.
Center of Excellence in Applied Medical Virology (CEAMV), Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand.
Sci Rep. 2025 Jan 4;15(1):781. doi: 10.1038/s41598-024-84833-y.
The pathogenic oomycete Pythium insidiosum causes a fatal infectious illness known as pythiosis, impacting humans and certain animals in numerous countries in the tropics and subtropics. Delayed diagnosis is a primary factor contributing to the heightened morbidity and mortality associated with the disease. Several new serodiagnostic methods have been developed to improve the identification of pythiosis. However, these assays provide only indirect evidence of pythiosis and are not readily available in the commercial market. Here, we have developed an affordable point-of-care test (POCT) kit based on an immunochromatographic assay to detect P. insidiosum antigens directly. Our recent findings reveal that the lateral flow sandwich immunological testing cassette can accurately identify vascular pythiosis antigens using a small volume of patient's plasma, accomplishing diagnostic efficiency, with a limit of detection (LOD) of 8 ng/mL. This prototype cartridge represents a significant stride toward advancing enriched POCT for pythiosis serodiagnosis.
致病性卵菌隐孢子虫可引发一种名为腐皮病的致命传染病，影响着热带和亚热带众多国家的人类和某些动物。诊断延误是导致该疾病发病率和死亡率升高的主要因素。为了改进腐皮病的诊断，已开发出几种新的血清诊断方法。然而，这些检测方法仅提供腐皮病的间接证据，且在商业市场上不易获得。在此，我们基于免疫层析法开发了一种经济实惠的即时检测（POCT）试剂盒，可直接检测隐孢子虫抗原。我们最近的研究结果表明，侧向流动夹心免疫检测试剂盒能够使用少量患者血浆准确识别血管腐皮病抗原，实现了诊断效率，检测限（LOD）为8 ng/mL。这种原型试剂盒代表了在推进用于腐皮病血清诊断的强化POCT方面迈出的重要一步。
