Shil Aparna, Mukherjee Sushmit, Biswas Prerona, Majhi Sudipta, Sikdar Sima, Bishayi Biswadev, Sikdar Née Bhakta Mausumi
Microbiology, Nutrition and Dietetics Laboratory, Physiology Unit, Department of Life Sciences, Presidency University, Kolkata 700073, India.
Department of Physiology, Immunology Laboratory, University of Calcutta, University Colleges of Science and Technology, Kolkata 700009, India.
Open Life Sci. 2023 Sep 26;18(1):20220718. doi: 10.1515/biol-2022-0718. eCollection 2023.
It is essential to revisit the global biodiversity, search for ethnopharmacologically relevant plants, and unveil their untapped potential to overcome the complications associated while treating infections triggered by multiple antibiotic-resistant . (L.) G. Don of the Apocynaceae family is a medicinal plant used for remedial purposes against infectious diseases from ancient times. In this study, we intended to evaluate the mechanism by which the ethanolic extract of . root (EECRR) causes the reversal of ampicillin resistance in . To achieve this goal, we have stained EECRR-treated with acridine orange, analysed DNA damage by comet assay, and studied the alteration of plasmid band pattern and expression of penicillin-binding protein 2a (PBP2a) protein. Experiments revealed better killing efficiency of EECRR at its minimum inhibitory concentration (MIC) doses due to DNA damage and reducing plasmid band intensities along with a decline in the expression of PBP2a in EECRR-treated cells at half-MIC dose. EECRR proved to be an efficient growth inhibitor of that reduces the expression of PBP2a. Therefore, EECRR can also render ampicillin-resistant susceptible to the antibiotic.
重新审视全球生物多样性、寻找具有民族药理学意义的植物并揭示其未开发的潜力以克服在治疗多重耐药菌引发的感染时所涉及的并发症至关重要。夹竹桃科的（L.）G. Don是一种药用植物，自古以来就用于治疗传染病。在本研究中，我们旨在评估……根乙醇提取物（EECRR）导致……氨苄西林耐药性逆转的机制。为实现这一目标，我们用吖啶橙对经EECRR处理的……进行染色，通过彗星试验分析DNA损伤，并研究质粒条带模式的变化以及青霉素结合蛋白2a（PBP2a）的表达。实验表明，在其最低抑菌浓度（MIC）剂量下，EECRR具有更好的杀菌效率，这是由于DNA损伤以及在半MIC剂量下，经EECRR处理的细胞中质粒条带强度降低以及PBP2a表达下降所致。EECRR被证明是一种有效的……生长抑制剂，可降低PBP2a的表达。因此，EECRR还可使耐氨苄西林的……对该抗生素敏感。
