Reyes-Umana Victor M, Coates John D
Department of Plant and Microbial Biology, University of California , Berkeley, California, USA.
Microbiol Spectr. 2023 Sep 29;11(5):e0091523. doi: 10.1128/spectrum.00915-23.
The genus is currently a non-validated taxon that has been identified in several recent publications as members of microbial communities arising from marine environments. Very little is known about the biology of spp., and no pure cultures are presently found in any culture collections. The current epitaph of was given to the organism under the assumption that all members of this genus are denitrifying bacteria. This study performs phenotypic and genomic analyses on three new spp. isolated from tidal mudflats in the San Francisco Bay. We demonstrate that spp. are indeed all facultative denitrifying bacteria that utilize a variety of carbon sources such as acetate, lactate, and succinate. In addition, individual strains also use the esoteric electron acceptors perchlorate, chlorate, and iodate. Both 16S and Rps/Rpl phylogenetic analyses place spp. as a deep branching clade in the family , separate from either spp., spp., or spp. Genome sequencing reveals a G + C content ranging from 63.72% to 66.54%, and genome sizes range between 4.39 and 5.18 Mb. Genes for salt tolerance and denitrification are distinguishing features that separate spp. from the closely related and genera. IMPORTANCE The genus is currently a non-validated taxon that has been identified in several recent publications as members of microbial communities arising from marine environments. Very little is known about the biology of spp., and no pure cultures are presently found in any culture collections. The current epitaph of was given to the organism under the assumption that all members of this genus are denitrifying bacteria. This study performs phenotypic and genomic analyses on three spp., sp. nov.-a novel iodate-reducing bacterium-and two novel perchlorate-reducing bacteria, and , isolated from San Francisco Bay intertidal mudflats.
该属目前是一个未经验证的分类单元，在最近的几篇出版物中被确定为源自海洋环境的微生物群落成员。关于该属物种的生物学特性知之甚少，目前在任何培养物保藏中心都未发现纯培养物。该属目前的描述是基于所有成员都是反硝化细菌这一假设给出的。本研究对从旧金山湾潮间带泥滩分离出的三个新的该属物种进行了表型和基因组分析。我们证明该属物种确实都是兼性反硝化细菌，它们利用多种碳源，如乙酸盐、乳酸盐和琥珀酸盐。此外，个别菌株还利用高氯酸盐、氯酸盐和碘酸盐等特殊电子受体。16S和Rps/Rpl系统发育分析均表明该属物种在该科中是一个深度分支的进化枝，与其他属的物种分开。基因组测序显示G + C含量在63.72%至66.54%之间，基因组大小在4.39至5.18 Mb之间。耐盐和反硝化基因是该属物种与密切相关的其他属区分开来的显著特征。重要性该属目前是一个未经验证的分类单元，在最近的几篇出版物中被确定为源自海洋环境的微生物群落成员。关于该属物种的生物学特性知之甚少，目前在任何培养物保藏中心都未发现纯培养物。该属目前的描述是基于所有成员都是反硝化细菌这一假设给出的。本研究对从旧金山湾潮间带泥滩分离出的三个该属物种、一个新的碘酸盐还原细菌新种以及两个新的高氯酸盐还原细菌进行了表型和基因组分析。
