Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, 145, Xingda Rd, Taichung, 40227, Taiwan.
Innovation and Development Center of Sustainable Agriculture, National Chung Hsing University, Taichung, Taiwan.
Antonie Van Leeuwenhoek. 2020 Jul;113(7):933-946. doi: 10.1007/s10482-020-01401-w. Epub 2020 Mar 28.
A polyphasic taxonomic approach was used to characterise two presumably novel bacteria, designated strains CC-YHH838 and CC-YHH848 isolated from termite nest and rhizosphere of Ficus religiosa, respectively. These two nitrogen-fixing strains were observed to be Gram-staining-negative, aerobic rod, and colonies were yellowish in color. Growth of strains was observed at 20-37 °C, pH 7-8, and in the presence of 1-2% NaCl. Phylogenetic analyses based on 16S rRNA genes revealed a distinct taxonomic position attained by strain CC-YHH838 and CC-YHH848 associated with Thauera hydrothermalis (97.1% sequence identity), and formed a separate branch with Azoarcus indigens (95.4%), Aromatoleum aromaticum (96.2%), and lower sequence similarity to other species. The calculation of OrthoANI values pointed out strains CC-YHH838 and CC-YHH848 gave 78.9% and 79.8% compared to Thauera hydrothermalis, respectively. The major fatty acids (> 5%) were C, C cyclo, C 3-OH, Cω7c/Cω6c and Cω7c/Cω6c. The polar lipid profile comprised phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and unidentified aminophospholipid and phospholipids; the predominant polyamines were putrescine and spermidine. The predominant respiratory system was ubiquinone (Q-8) and the DNA G + C contents were 61.4 ± 0.1 mol% and 60.2 ± 1.3 mol%, respectively. Based on the phylogenetic and polyphasic comparisons, strains CC-YHH838 and CC-YHH848 are proposed to represent two novel species within the genus Azoarcus in the family Rhodocyclaceae, for which the name Azoarcus nasutitermitis sp. nov. (type strain CC-YHH838 = BCRC 81059 = JCM 32001) and Azoarcus rhizosphaerae sp. nov. (type strain CC-YHH848 = BCRC 81060 = JCM 32002) were proposed.
采用多相分类法对两株可能的新型细菌进行了表征，分别命名为 CC-YHH838 和 CC-YHH848 菌株，它们分别从白蚁巢和榕树根际中分离得到。这两株固氮菌株为革兰氏阴性、需氧杆状菌，菌落呈黄色。菌株的生长温度为 20-37°C，pH 值为 7-8，在 1-2%NaCl 存在的情况下生长。基于 16S rRNA 基因的系统发育分析显示，CC-YHH838 和 CC-YHH848 菌株与 Thauera hydrothermalis 密切相关（97.1%序列同一性），并与 Azoarcus indigens（95.4%）、Aromatoleum aromaticum（96.2%）形成单独的分支，与其他物种的序列相似性较低。Orthogonal Average Nucleotide Identity (OrthoANI) 值的计算表明，CC-YHH838 和 CC-YHH848 菌株与 Thauera hydrothermalis 的相似度分别为 78.9%和 79.8%。主要脂肪酸（>5%）为 C、C 环、C3-OH、Cω7c/Cω6c 和 Cω7c/Cω6c。极性脂谱由磷脂酰乙醇胺、磷脂酰甘油、双磷脂酰甘油和未鉴定的氨基磷脂和磷脂组成；主要多胺为腐胺和亚精胺。主要呼吸系统是泛醌（Q-8），DNA G+C 含量分别为 61.4±0.1mol%和 60.2±1.3mol%。基于系统发育和多相比较，CC-YHH838 和 CC-YHH848 菌株被提议代表 Rhodocyclaceae 家族 Azoarcus 属中的两个新型物种，分别命名为 Azoarcus nasutitermitis sp. nov.（模式菌株 CC-YHH838=BCRC 81059=JCM 32001）和 Azoarcus rhizosphaerae sp. nov.（模式菌株 CC-YHH848=BCRC 81060=JCM 32002）。
