Laurent C, Hellström S, Appelgren L E, Sehlin J, Lilja K
Am J Otolaryngol. 1986 Sep-Oct;7(5):334-40. doi: 10.1016/s0196-0709(86)80021-7.
The fate of exogenous high-molecular-weight hyaluronic acid applied in the middle ear was studied in rats. Tritium-labelled hyaluronic acid was deposited, and its disappearance through the eustachian tube was followed by analysis of nasopharyngeal secretions. Radioactivity in the secretions reached a peak after three hours and approached zero after 12 hours, indicating that almost all hyaluronic acid had been removed. The middle ears were all empty when opened 24 hours after the deposition of hyaluronic acid. The fate of hyaluronic acid after obstruction of the eustachian tube was followed both with radioactive hyaluronic acid and subsequent autoradiography and by direct analysis of the hyaluronic acid concentration and the hyaluronic acid molecular weight distribution. Radioactive hyaluronic acid was confined to the obstructed middle ear, and no uptake into surrounding tissues could be detected between three hours and four days after the deposition. The amount of hyaluronic acid deposited could be recovered to about the same extent for up to six days. Analysis of the molecular weight distribution of the deposited material indicated only a slow degradation, if any, during the same time period.
研究了大鼠中耳应用外源性高分子量透明质酸后的转归。沉积了氚标记的透明质酸，并通过分析鼻咽分泌物追踪其经咽鼓管的消失情况。分泌物中的放射性在3小时后达到峰值，12小时后接近零，表明几乎所有透明质酸均已清除。在透明质酸沉积24小时后打开中耳时，中耳均为空的。通过放射性透明质酸及随后的放射自显影以及直接分析透明质酸浓度和透明质酸分子量分布，追踪了咽鼓管阻塞后透明质酸的转归。放射性透明质酸局限于阻塞的中耳，在沉积后3小时至4天内未检测到其被周围组织摄取。在长达6天的时间内，沉积的透明质酸量可在大致相同程度上回收。对沉积物质的分子量分布分析表明，在同一时间段内，即使有降解，也很缓慢。
