Wu Ruibang, Liu Zheng, Huang Yong, Huang Leizhen, Wang Juehan, Ding Hong, Wang Zhe, Li Qiujiang, Zhu Ce, Liu Limin, Zhang Li, Feng Ganjun
Department of Orthopedics Surgery and Orthopedic Research Institute, Analytical & Testing Center, West China Hospital, Sichuan University, Chengdu 610065, China.
Bioconjug Chem. 2023 Oct 18;34(10):1902-1913. doi: 10.1021/acs.bioconjchem.3c00403. Epub 2023 Sep 29.
The emergence of nanozymes presents a promising alternative to antibiotics for reactive oxygen species-mediated broad-spectrum antimicrobial purposes, but nanozymes still face challenges of low therapeutic efficiency and poor biocompatibility. Herein, we creatively prepared a novel kind of hollow cobalt sulfide (CoS) nanospheres with a unique mesoporous structure that is able to provide numerous active sites for enzyme-like reactions. The results revealed that 50 μg/mL of CoS nanospheres exhibited strong peroxidase- and oxidase-like activities under physiological conditions with the assistance of a low concentration of hydrogen peroxide (HO, 100 μM) while possessing highly efficient GSH-depletion ability, which endowed CoS nanospheres with triple enzyme-like properties to combat bacterial infections. The in vitro experiments demonstrated that the CoS nanozyme displayed significant antibacterial effects against both Gram-positive () and Gram-negative (). The in vivo implantation showed that the synthesized CoS effectively eliminated bacteria and promoted the recovery of infected wounds in rats while exhibiting a low cytotoxicity. This study provides a promising treatment strategy to accelerate infected wound healing.
纳米酶的出现为活性氧介导的广谱抗菌目的提供了一种有前景的抗生素替代方案，但纳米酶仍面临治疗效率低和生物相容性差的挑战。在此，我们创造性地制备了一种新型的具有独特介孔结构的中空硫化钴（CoS）纳米球，该结构能够为类酶反应提供大量活性位点。结果表明，50μg/mL的CoS纳米球在低浓度过氧化氢（HO，100μM）的辅助下，在生理条件下表现出强大的过氧化物酶和氧化酶样活性，同时具有高效的谷胱甘肽消耗能力，这赋予了CoS纳米球三重类酶特性以对抗细菌感染。体外实验表明，CoS纳米酶对革兰氏阳性菌（）和革兰氏阴性菌（）均显示出显著的抗菌效果。体内植入实验表明，合成的CoS能有效消除细菌并促进大鼠感染伤口的恢复，同时表现出低细胞毒性。本研究提供了一种有前景的治疗策略，以加速感染伤口的愈合。
