Laboratory of Endocrine System Development, A. P. Avtsyn Research Institute of Human Morphology, A. P. Petrovsky Russian Research Center of Surgery, Moscow, Russia.
Bull Exp Biol Med. 2023 Aug;175(4):549-556. doi: 10.1007/s10517-023-05903-1. Epub 2023 Sep 30.
Regulation of morphogenetic processes during postnatal development of the rat adrenal medulla was studied. Termination of the adrenal medulla growth was found to be associated with decreased chromaffin cell proliferation, activation of canonical Wnt-signaling pathway, and enhanced expression of Sonic Hedgehog ligand. Analysis of transcription factors associated with pluripotency revealed increased percentage of Oct4-expressing cells by the end of medulla growth and no signs of Sox2 expression. All the cells demonstrating activation of Wnt-signaling and expression of Oct4 and Sonic Hedgehog were found to be highly differentiated chromaffin cells actively producing tyrosine hydroxylase. These findings allow considering the formation of the cell pools for dedifferentiation as a putative mechanism for physiological regeneration of the adrenal medulla.
研究了大鼠肾上腺髓质出生后发育过程中形态发生过程的调节。发现肾上腺髓质生长的终止与嗜铬细胞增殖减少、经典 Wnt 信号通路的激活以及 Sonic Hedgehog 配体的表达增强有关。与多能性相关的转录因子分析显示，在髓质生长结束时，Oct4 表达细胞的百分比增加，而 Sox2 表达的迹象不存在。所有表现出 Wnt 信号激活和 Oct4 和 Sonic Hedgehog 表达的细胞均被发现是高度分化的嗜铬细胞，它们正在积极产生酪氨酸羟化酶。这些发现使人们认为形成去分化细胞池是肾上腺髓质生理再生的一种潜在机制。
