Department of Molecular Pharmacology and Physiology, Morsani College of Medicine, University of South Florida, Tampa, FL, USA.
Methods Mol Biol. 2024;2711:241-256. doi: 10.1007/978-1-0716-3429-5_20.
Endothelial bioenergetics have emerged as a key regulator of endothelial barrier function. Glycolytic parameters have been linked to barrier enhancement, and interruption with mitochondrial complexes was shown to disrupt endothelial barrier. Therefore, a new technology that has been introduced to assess bioenergetics and metabolism has also made it possible to determine roles of specific energy production pathways in endothelial health. The Seahorse extracellular flux analysis by Agilent technologies is a state of the art tool that has been more frequently used to evaluate bioenergetics of endothelial cells. This chapter includes details about different assays that can be used to study endothelial cells using the Seahorse analyzer and how interpretation of the results can provide novel insight about endothelial metabolism.
内皮细胞生物能量学已成为内皮屏障功能的关键调节因子。糖酵解参数与屏障增强有关，而线粒体复合物的中断被证明会破坏内皮屏障。因此，一种新的评估生物能量学和代谢的技术也使得确定特定能量产生途径在血管内皮健康中的作用成为可能。安捷伦科技公司的 Seahorse 细胞外通量分析是一种先进的工具，它越来越多地被用于评估内皮细胞的生物能量学。本章包括有关使用 Seahorse 分析仪研究内皮细胞的不同测定法的详细信息，以及如何解释结果可以提供有关内皮代谢的新见解。
