Paediatric Dentistry, Faculty of Dentistry, Prince Philip Dental Hospital, The University of Hong Kong, 34 Hospital Road, Sai Ying Pun, Hong Kong, China; Dental Biomaterials, Faculty of Dental Medicine, Al-Azhar University, Cairo, Egypt.
Paediatric Dentistry, Faculty of Dentistry, Prince Philip Dental Hospital, The University of Hong Kong, 34 Hospital Road, Sai Ying Pun, Hong Kong, China.
J Dent. 2023 Nov;138:104731. doi: 10.1016/j.jdent.2023.104731. Epub 2023 Sep 29.
To investigate enamel remineralization and antimicrobial effect of sodium fluoride (NaF) varnish containing calcium strontium silicate (CSR).
CSR was synthesized by sol-gel process and incorporated in 5 % NaF varnish at three different concentrations (1 %, 2 %, and 4 % w/v). The treatment/control groups were: 1 % CSR+NaF, 2 % CSR+NaF, 4 % CSR+NaF, NaF, and no treatment. Strontium and fluoride release from the varnishes was evaluated. Sound enamel specimens (n = 6) were demineralized, varnish-treated, and subjected to remineralization cycle. Mineral density of enamel specimens was evaluated using micro-CT. Antimicrobial effect of the varnishes on Streptococcus mutans and Lactobacillus acidophilus biofilms was assessed using confocal laser scanning microscopy. The HGF-1 cytotoxicity of the varnishes was examined using CCK-8 assay.
Both 2 % and 4 % CSR+NaF varnishes showed significantly higher F release and remineralization potential than NaF varnish (p < 0.05). Dead bacterial proportion of 4 % CSR+NaF varnish was significantly higher than NaF varnish (p < 0.05). The CFUs values of both S. mutans and L. acidophilus were significantly lower in 4 % CSR+NaF group than NaF group (p < 0.05). No significant difference in cell viability was observed among the groups (p > 0.05).
Incorporation of 4 % CSR in a NaF varnish significantly enhanced its enamel remineralization and antimicrobial potential with no cytotoxic effect.
Dental caries is a major public health problem globally. The study highlights the great potential of CSR-doped NaF varnish as a novel anti-caries agent with synergistic remineralizing and antimicrobial properties to combat early enamel caries lesions in the general population.
研究硅酸锶钙（CSR）氟化物（NaF）水门汀的再矿化和抗菌效果。
采用溶胶-凝胶法合成 CSR，并将其以 3 种不同浓度（1%、2%和 4%w/v）掺入 5%NaF 水门汀中。处理/对照组为：1%CSR+NaF、2%CSR+NaF、4%CSR+NaF、NaF 和无处理。评估水门汀中锶和氟的释放情况。将无龋釉质标本（n=6）脱矿，涂覆水门汀，并进行再矿化循环。使用 micro-CT 评估釉质标本的矿物质密度。使用共聚焦激光扫描显微镜评估水门汀对变形链球菌和嗜酸乳杆菌生物膜的抗菌效果。使用 CCK-8 法检测水门汀的 HGF-1 细胞毒性。
2%和 4%CSR+NaF 水门汀的 F 释放和再矿化潜力均显著高于 NaF 水门汀（p<0.05）。4%CSR+NaF 水门汀的死菌比例显著高于 NaF 水门汀（p<0.05）。4%CSR+NaF 组的变形链球菌和嗜酸乳杆菌 CFU 值均显著低于 NaF 组（p<0.05）。各组细胞活力无显著差异（p>0.05）。
将 4%CSR 掺入 NaF 水门汀可显著增强其再矿化和抗菌潜力，同时无细胞毒性。
龋齿是全球主要的公共卫生问题。该研究突出了 CSR 掺杂 NaF 水门汀作为一种新型抗龋剂的巨大潜力，具有协同的再矿化和抗菌特性，可用于对抗普通人群的早期釉质龋损。
