Post-doctoral Fellow in Paediatric Dentistry, Faculty of Dentistry, The University of Hong Kong, Hong Kong.
Intern/Research Assistant, Paediatric Dentistry, Department of Clinical Sciences, College of Dentistry, Ajman University, Ajman, United Arab Emirates.
J Dent. 2024 Sep;148:104965. doi: 10.1016/j.jdent.2024.104965. Epub 2024 Mar 26.
The study objectives were to examine the physical properties and enamel remineralization potential of fluoride (F) varnishes incorporated with arginine (Arg).
Four commercial F varnishes: 1) Duraphat®; 2) Flúor Protector®, 3) Fluor Protector S®, and 4) Fluorimax™ were supplemented with 2% w/v. Arg. The control/experimental varnishes underwent rheometric analysis to assess varnish density (δ), velocity (ν), and associated viscosity, both quantitatively (ν/δ) and qualitatively based on determined mass, volume, distance flow, and time under experimentation. The varnish wet/dry weights (at 2 h) were also analysed. Further, sound enamel specimens (T0) with artificial incipient caries-like lesions (T1) were treated with control/experimental varnishes and subjected to remineralization assay with artificial saliva for 6 h. Thereafter (T2), the specimens were characterized to estimate precipitated Ca and net enamel F uptake. Additionally, mineral density (MD) was assessed using micro-CT at T0, T1, and T2 to derive mineral gain (MG) and % remineralization for the treatment groups.
When Arg is incorporated, the physical properties of the F-containing varnishes undergo a significant transformation, resulting in higher density, varnish weight, dry varnish weight, and viscosity compared to their respective control varnishes (p < 0.05). Incorporating Arg-in Duraphat®, Fluor Protector S®, and Fluorimax™ significantly improved both enamel Ca precipitation and F uptake compared to the respective controls (p < 0.05). Additionally, the enamel F uptake was significantly higher with all the tested varnishes when enriched with Arg (p < 0.05). The combined data for MD, MG, and % remineralization suggests that the remineralization potential of F-varnishes significantly increased when enriched with Arg (p < 0.05).
Incorporating Arg in inorganic F varnishes improves their physical properties and enhances the enamel remineralization potential of the varnishes.
This study highlights the possibility of incorporating Arg in distinct F-source varnishes. The synergism between active components (Arg-F) aids in enhanced remineralization and superior varnish physical properties, demonstrating a promising approach for high caries-risk patients.
本研究旨在探究含精氨酸（Arg）的氟化物（F）涂料的物理性能和再矿化潜力。
四种商业 F 涂料：1）Duraphat®；2）Flúor Protector®；3）Fluor Protector S®；4）Fluorimax™中添加 2%w/v 的 Arg。对对照/实验涂料进行流变分析，以评估涂料密度（δ）、速度（ν）以及相关的定量（ν/δ）和定性粘度，根据实验中确定的质量、体积、距离流量和时间进行评估。还分析了涂料的湿/干重量（2 h 时）。此外，对具有人工初期龋样病变的健康牙釉质标本（T0）用对照/实验涂料进行处理，并在人工唾液中进行再矿化试验 6 h。之后（T2），对标本进行特征分析，以评估沉淀的 Ca 和净牙釉质 F 摄取量。此外，使用 micro-CT 在 T0、T1 和 T2 时评估矿化密度（MD），以获得治疗组的矿化增量（MG）和再矿化率（%）。
当 Arg 被掺入时，含氟涂料的物理性能发生显著变化，与相应的对照涂料相比，其密度、涂料重量、干涂料重量和粘度均显著增加（p<0.05）。与相应的对照涂料相比，在 Duraphat®、Fluor Protector S®和 Fluorimax™中掺入 Arg 显著提高了牙釉质 Ca 沉淀和 F 摄取（p<0.05）。此外，当所有测试的涂料都富含 Arg 时，牙釉质 F 的摄取量显著增加（p<0.05）。MD、MG 和再矿化率的综合数据表明，当 Arg 丰富时，F 涂料的再矿化潜力显著增加（p<0.05）。
在无机 F 涂料中掺入 Arg 可改善其物理性能，并增强涂料的牙釉质再矿化潜力。
本研究强调了在不同 F 源涂料中掺入 Arg 的可能性。活性成分（Arg-F）之间的协同作用有助于增强再矿化和改善涂料的物理性能，为高龋风险患者提供了一种有前途的方法。
