Department of Restorative Dentistry, Sao Paulo State University - UNESP, Av. Eng. Francisco Jose Longo, 777, Sao Jose dos Campos, SP, Brazil.
Department of Restorative Dentistry, Sao Paulo State University - UNESP, Av. Eng. Francisco Jose Longo, 777, Sao Jose dos Campos, SP, Brazil.
J Dent. 2022 Apr;119:104074. doi: 10.1016/j.jdent.2022.104074. Epub 2022 Feb 24.
To evaluate the remineralizing effect of varnishes containing different concentrations of S-PRG filler or sodium fluoride on enamel caries lesions.
Enamel specimens were obtained from bovine incisors, flattened and polished. Half of the surface was protected, and artificial caries lesion was created in the unprotected area. They were assigned into six groups (n=20) according to the varnish applied: four containing S-PRG filler with different concentrations (10%, 20%, 30% and 40% w/w), one containing 5% sodium fluoride (5%NaF) as positive control, and one without treatment as a negative control (NC). Varnishes were applied on specimen's caries-affected surface and removed after 24 h. Varnish application and pH cycling (8 days) were performed twice. Cross-sectional microhardness was measured (10-100 µm depth). Knoop microhardness percentage (KHN%) of caries affected/treated area in relation to the sound enamel area at the same depth was calculated. The area under the curve (AUC) and the Δ were also calculated. Statistical analysis was performed by two-way ANOVA for KHN% and one-way ANOVA for Δ, followed by Tukey test (α=5%).
Significant differences were found for the factors "treatment" (p=0.0001) and "depth" (p=0001). For the factor "treatment", the results of the Tukey test were: NC-62.2(10.3)a, 10%S-PRG-69.2(9.3)b, 20%S-PRG-71.8(9.7)bc, 30%S-PRG-74.5(9.4)c, 5%NaF-79.6(8.7)d, 40%S-PRG-85.6(8.4)e. In relation to Δ, significant differences were observed among the groups (p=0.0001) and better results for 40% S-PRG and 5%NaF.
The varnish containing 40% S-PRG fillers was the most effective to promote the enamel caries remineralization, followed by the one containing 5% sodium fluoride.
The varnish containing 40% of S-PRG fillers represents an alternative for initial enamel caries remineralization, since it was more effective than the conventional 5% NaF based product.
评估含有不同浓度 S-PRG 填料或氟化钠的釉质涂料对牙釉质龋损的再矿化效果。
从牛切牙获得釉质标本，使其平坦并抛光。一半的表面被保护，未保护区域形成人工龋损。根据所涂的釉质涂料，将它们分为六组（n=20）：四种含有不同浓度的 S-PRG 填料（10%、20%、30%和 40%w/w），一种含有 5%氟化钠（5%NaF）作为阳性对照，一种不做任何处理作为阴性对照（NC）。将涂料涂在标本的龋损表面，24 小时后去除。进行两次涂料应用和 pH 循环（8 天）。测量横截面显微硬度（10-100 µm 深度）。计算受龋损/处理区域相对于相同深度的正常釉质区域的克努普显微硬度百分比（KHN%）。还计算了曲线下面积（AUC）和Δ。用双向方差分析（KHN%）和单向方差分析（Δ）对数据进行分析，然后用 Tukey 检验（α=5%）进行比较。
“处理”因素（p=0.0001）和“深度”因素（p=0.0001）有显著差异。对于“处理”因素，Tukey 检验的结果为：NC-62.2（10.3）a、10%S-PRG-69.2（9.3）b、20%S-PRG-71.8（9.7）bc、30%S-PRG-74.5（9.4）c、5%NaF-79.6（8.7）d、40%S-PRG-85.6（8.4）e。关于Δ，组间有显著差异（p=0.0001），40%S-PRG 和 5%NaF 的效果更好。
含有 40%S-PRG 填料的釉质涂料在促进牙釉质再矿化方面最有效，其次是含有 5%氟化钠的釉质涂料。
含有 40%S-PRG 填料的釉质涂料是初始牙釉质再矿化的一种替代选择，因为它比传统的 5%NaF 产品更有效。
