Functional Microbe Technology Group, Department of Biological and Chemical Engineering, Aarhus University, Gustav Wieds Vej 10, 8000 Aarhus, Denmark.
Center for Innovative Food (CiFOOD), Department of Food Science, Aarhus University, AgroFood Park 48, 9200 Aarhus N, Denmark.
FEMS Microbiol Ecol. 2023 Sep 19;99(10). doi: 10.1093/femsec/fiad107.
Algae are a rich but unexplored source of fibers with the potential to contribute to the next generation of prebiotics. The sulfated brown algae polysaccharide, fucoidan, is mainly composed of the deoxy-hexose L-fucose, which can be metabolized to 1,2-propanediol (1,2-PD) or lactate by gut microbes as precursors of propionate and butyrate. It was the aim of this study to investigate the impact of fucoidan on the fermentation capacity of the fecal microbiota and to compare to fucose. In batch fermentations of fecal microbiota collected from 17 donor samples, fucose promoted the production of propionate while no consistent effect was observed for commercial fucoidan and Fucus vesiculosus extract prepared in this study containing laminarin and fucoidan. H2S production was detected under all tested conditions, and levels were significantly lower in the presence of fucose in a dose-dependent manner. The addition of high fucose levels led to higher relative abundance of microbial 1,2-PD and lactate cross-feeders. Our results highlight that fucose and not fucoidan addition impacted fermentation capacity and increased the proportions of propionate and butyrate, which allows for precise modulation of intestinal microbiota activity.
藻类是一种丰富但尚未开发的纤维来源，具有成为下一代益生元的潜力。硫酸化褐藻多糖——褐藻糖胶，主要由脱氧己糖 L-岩藻糖组成，可被肠道微生物代谢为 1,2-丙二醇（1,2-PD）或乳酸，作为丙酸和丁酸的前体。本研究旨在探讨褐藻糖胶对粪便微生物群发酵能力的影响，并与岩藻糖进行比较。在对来自 17 个供体样本的粪便微生物群进行的分批发酵中，岩藻糖促进了丙酸的产生，而商业褐藻糖胶和本研究中用褐藻制备的含有昆布多糖和褐藻糖胶的提取物没有表现出一致的效果。在所有测试条件下均检测到 H2S 的产生，并且在存在岩藻糖的情况下，H2S 的产生水平呈剂量依赖性显著降低。添加高岩藻糖水平导致微生物 1,2-PD 和乳酸交叉喂养者的相对丰度增加。我们的研究结果强调，岩藻糖而不是褐藻糖胶的添加影响了发酵能力，并增加了丙酸和丁酸的比例，这使得肠道微生物群活性可以得到精确调节。
