Chen M, Hickey P J
Appl Environ Microbiol. 1986 Oct;52(4):778-81. doi: 10.1128/aem.52.4.778-781.1986.
Two holding media were compared for their effects on total coliform recovery by the delayed-incubation membrane filter procedure. LES-MF holding medium contains tryptone, m-Endo broth, dipotassium hydrogen phosphate, sodium benzoate, sulfanilamide, para-aminobenzoic acid, and cycloheximide (pH 7.0). m-ST holding medium contains ethanol, sodium monophosphate, dipotassium hydrogen phosphate, sulfanilamide, and Tris (pH 8.6). In tests with natural water and wastewater samples from various sources, recovery with LES-MF and m-ST were similar after a 1-day holding period. With LES-MF, however, after a 2- or 3-day holding period, coliform bacteria frequently were partially or totally overgrown by noncoliforms, causing significant reductions in coliform counts. No significant overgrowth was observed with m-ST. We propose that m-ST be used for all holding periods longer than 1 day.
通过延迟培养膜过滤法，比较了两种保存培养基对总大肠菌群回收率的影响。LES-MF保存培养基含有胰蛋白胨、m-远藤氏肉汤、磷酸氢二钾、苯甲酸钠、磺胺、对氨基苯甲酸和环己酰亚胺（pH 7.0）。m-ST保存培养基含有乙醇、磷酸二氢钠、磷酸氢二钾、磺胺和三羟甲基氨基甲烷（pH 8.6）。在对来自各种来源的天然水和废水样本进行的测试中，经过1天的保存期后，LES-MF和m-ST的回收率相似。然而，使用LES-MF时，经过2天或3天的保存期后，大肠菌群细菌经常被非大肠菌群部分或完全过度生长，导致大肠菌群计数显著减少。使用m-ST未观察到明显的过度生长。我们建议，对于所有超过1天的保存期，均使用m-ST。
