Grabow W O, du Preez M
Appl Environ Microbiol. 1979 Sep;38(3):351-8. doi: 10.1128/aem.38.3.351-358.1979.
Total coliform counts obtained by means of standard membrane filtration techniques, using MacConkey agar, m-Endo LES agar, Teepol agar, and pads saturated with Teepol broth as growth media, were compared. Various combinations of these media were used in tests on 490 samples of river water and city wastewater after different stages of conventional purification and reclamation processes including lime treatment, and filtration, active carbon treatment, ozonation, and chlorination. Endo agar yielded the highest average counts for all these samples. Teepol agar generally had higher counts then Teepol broth, whereas MacConkey agar had the lowest average counts. Identification of 871 positive isolates showed that Aeromonas hydrophila was the species most commonly detected. Species of Escherichia, Citrobacter, Klebsiella, and Enterobacter represented 55% of isolates which conformed to the definition of total coliforms on Endo agar, 54% on Teepol agar, and 45% on MacConkey agar. Selection for species on the media differed considerably. Evaluation of these data and literature on alternative tests, including most probable number methods, indicated that the technique of choice for routine analysis of total coliform bacteria in drinking water is membrane filtration using m-Endo LES agar as growth medium without enrichment procedures or a cytochrome oxidase restriction.
比较了通过标准膜过滤技术，使用麦康凯琼脂、m - 远藤LES琼脂、替波尔琼脂以及用替波尔肉汤饱和的垫作为生长培养基获得的总大肠菌群数。这些培养基的各种组合用于对490个河水和城市污水样本进行测试，这些样本经过了常规净化和回收过程的不同阶段，包括石灰处理、过滤、活性炭处理、臭氧化和氯化。远藤琼脂对所有这些样本产生的平均计数最高。替波尔琼脂的计数通常高于替波尔肉汤，而麦康凯琼脂的平均计数最低。对871个阳性分离株的鉴定表明，嗜水气单胞菌是最常检测到的物种。大肠埃希氏菌、柠檬酸杆菌、克雷伯氏菌和肠杆菌属的物种占符合远藤琼脂上总大肠菌群定义的分离株的55%，替波尔琼脂上为54%，麦康凯琼脂上为45%。在这些培养基上对物种的选择有很大差异。对这些数据以及包括最可能数法在内的替代测试的文献评估表明，饮用水中总大肠菌群细菌常规分析的首选技术是使用m - 远藤LES琼脂作为生长培养基且无需富集程序或细胞色素氧化酶限制的膜过滤法。
