National, Reference Laboratory of Veterinary Drug Residues, South China Agriculture University, Guangzhou, China.
Guangdong Wenshi Dahuanong Biological Technology Co., Ltd, Yunfu, China.
J Food Sci. 2023 Nov;88(11):4773-4783. doi: 10.1111/1750-3841.16753. Epub 2023 Oct 1.
Fluoroquinolones are commonly used in poultry breeding. Few studies have evaluated the causes of serious drug residues in black-boned silky fowl until enrofloxacin has been banned in black-boned silky fowl breeding in the Chinese Veterinary Commission of Chinese Veterinary Pharmacopoeia (2020). However, similarly structured fluoroquinolones have not been studied in black-boned silky fowl. In this study, the elimination of tissue residues of danofloxacin (DAN) and difloxacin (DIF) was studied in four tissues of black-boned silky fowl. The specific administration methods were 100 mg/L of DIF aqueous solution for free drinking for 5 days and 50 mg/L of DAN aqueous solution for free drinking for 3 days. Based on the experiment, the withdrawal times of 44 days for muscle, 95 days for skin + fat, 3 days for liver, and 44 days for kidney of DAN were acquired, of 43 days for muscle, 61 days for skin + fat, 0 days for liver, and 38 days for kidney of DIF were acquired, which showed that DIF and DAN should be used with caution for application in black-boned silky fowl. In vitro experiments showed that black-boned silky fowl tissues had stronger adsorption capacity to DAN and DIF than yellow chicken tissues (especially in skin + fat), and melanin has a strong adsorption effect on DAN and DIF, which is an important reason for the high residual concentrations of fluoroquinolone in black-boned silky fowl.
氟喹诺酮类药物在禽类养殖中被广泛使用。直到中国兽医药品监察所《中国兽药典》（2020 年版）禁止在乌骨鸡养殖中使用恩诺沙星，才有少数研究评估了乌骨鸡中严重药物残留的原因。然而，同样结构的氟喹诺酮类药物在乌骨鸡中尚未被研究过。在这项研究中，研究了丹诺沙星（DAN）和二氟沙星（DIF）在乌骨鸡四个组织中的组织残留消除情况。具体给药方法为：自由饮用 5 天 100mg/L 的 DIF 水溶液和自由饮用 3 天 50mg/L 的 DAN 水溶液。基于实验，得出了 DAN 的肌肉停药时间为 44 天，皮肤+脂肪停药时间为 95 天，肝脏停药时间为 3 天，肾脏停药时间为 44 天；DIF 的肌肉停药时间为 43 天，皮肤+脂肪停药时间为 61 天，肝脏停药时间为 0 天，肾脏停药时间为 38 天，这表明 DIF 和 DAN 在乌骨鸡中的应用应谨慎。体外实验表明，乌骨鸡组织对 DAN 和 DIF 的吸附能力强于黄鸡组织（尤其是皮肤+脂肪），而黑色素对 DAN 和 DIF 有很强的吸附作用，这是氟喹诺酮类药物在乌骨鸡中残留浓度较高的一个重要原因。
