Hart L E, Schnoes H K, DeLuca H F
Arch Biochem Biophys. 1986 Nov 1;250(2):426-34. doi: 10.1016/0003-9861(86)90745-9.
Vitamin D-deficient laying hens were repleted with 25-hydroxy[26,27-3H]vitamin D3 or 1,25-dihydroxy[26,27-3H]vitamin D3. Egg production returned to normal for both groups of hens by the third week. Eggs from hens fed either 25-hydroxy[26,27-3H]vitamin D3 or 1,25-dihydroxy[26,27-3H]vitamin D3 contained 1,25-dihydroxy[26,27-3H]vitamin D3. Eggs from hens fed 25-hydroxy[26,27-3H]vitamin D3 contained substantial amounts of 25-hydroxy[26,27-3H]vitamin D3, while those from hens fed 1,25-dihydroxy[26,27-3H]vitamin D3 contained none. Plasma from 18-day embryos from hens fed 1,25-dihydroxy[26,27-3H]vitamin D3 contained little or no 1,25-dihydroxy[26,27-3H]vitamin D3, while that from 18-day embryos from hens given 25-hydroxy[26,27-3H]vitamin D3 had normal levels of 1,25-dihydroxy[26,27-3H]vitamin D3. No eggs from hens fed 1,25-dihydroxy[26,27-3H]vitamin D3 hatched, while eggs from hens fed 25-hydroxy[26,27-3H]vitamin D3 achieved a hatchability of 90%. It appears that embryos from hens maintained on 1,25-dihydroxyvitamin D3 as their sole source of vitamin D are essentially vitamin D deficient.
给维生素D缺乏的产蛋母鸡补充25-羟基[26,27-3H]维生素D3或1,25-二羟基[26,27-3H]维生素D3。到第三周时，两组母鸡的产蛋量均恢复正常。喂食25-羟基[26,27-3H]维生素D3或1,25-二羟基[26,27-3H]维生素D3的母鸡所产鸡蛋中含有1,25-二羟基[26,27-3H]维生素D3。喂食25-羟基[26,27-3H]维生素D3的母鸡所产鸡蛋含有大量的25-羟基[26,27-3H]维生素D3，而喂食1,25-二羟基[26,27-3H]维生素D3的母鸡所产鸡蛋则不含该物质。喂食1,25-二羟基[26,27-3H]维生素D3的母鸡所产18日龄胚胎的血浆中几乎不含或不含1,25-二羟基[26,27-3H]维生素D3，而喂食25-羟基[26,27-3H]维生素D3的母鸡所产18日龄胚胎的血浆中1,25-二羟基[26,27-3H]维生素D3水平正常。喂食1,25-二羟基[26,27-3H]维生素D3的母鸡所产鸡蛋均未孵化，而喂食25-羟基[26,27-3H]维生素D3的母鸡所产鸡蛋孵化率达90%。看来，以1,25-二羟基维生素D3作为唯一维生素D来源的母鸡所产胚胎基本上缺乏维生素D。
