Sunde M L, Turk C M, DeLuca H F
Science. 1978 Jun 2;200(4345):1067-9. doi: 10.1126/science.206963.
Laying hens maintained on 1,25-dihydroxyvitamin D3 as their sole source of vitamin D produce eggs which appear normal but which produce embryos having a defective upper mandible and which die at 18 to 19 days of embryonic life. Hens maintained on 25-hydroxyvitamin D3, on the other hand, produce normal embryos. Hens fed a vitamin D deficient diet produce eggs which develop the same embryonic defect. Injection of the affected eggs from the 1,25-dihydroxyvitamin D3 fed hens with vitamin D3, 25-hydroxyvitamin D3, or 1,25-dihydroxyvitamin D3 greatly increases the percentage of normal embryos. It therefore appears that 1,25-dihydroxyvitamin D3 is not transferred from hen to egg in sufficient amounts to support embryonic development and that vitamin D or its metabolites, or both, are necessary for normal chick embryo development.
以1,25 - 二羟基维生素D3作为唯一维生素D来源饲养的产蛋母鸡所产的蛋看似正常，但孵出的胚胎上颚有缺陷，且在胚胎发育18至19天时死亡。另一方面，以25 - 羟基维生素D3饲养的母鸡产出的胚胎正常。喂食缺乏维生素D日粮的母鸡所产的蛋会出现同样的胚胎缺陷。给喂食1,25 - 二羟基维生素D3的母鸡所产的受影响的蛋注射维生素D3、25 - 羟基维生素D3或1,25 - 二羟基维生素D3，可显著提高正常胚胎的比例。因此，似乎1,25 - 二羟基维生素D3从母鸡转移到蛋中的量不足以支持胚胎发育，维生素D或其代谢产物，或两者都对正常雏鸡胚胎发育是必需的。
