基于单细胞和批量RNA测序及实验分析验证桥粒芯胶蛋白-2在骨肉瘤中的作用。

Validation the role of desmocollin-2 in osteosarcoma based on single cell and bulk RNA seq and experimental analyses.

作者信息

Zeng Jiaxing, Sun Yu, Man Yunan, Tang Haijun, Xie Long, He Maolin

机构信息

Department of Spinal Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi, China.

Department of Traumatic Surgery & Microsurgery & Hand Surgery, Guangxi Zhuang Autonomous Region People's Hospital, Nanning, 530021, Guangxi, China.

出版信息

J Cancer. 2023 Aug 21;14(14):2619-2632. doi: 10.7150/jca.87411. eCollection 2023.

DOI:10.7150/jca.87411

PMID:37779876

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10539388/

Abstract

The aetiology of osteosarcoma (OS) remains unclear. Desmocollin-2 (DSC2) mediates intercellular adhesion and is involved in tumour progression. Therefore, we aim to investigate the potential role of DSC2 in OS. We analyzed the expression, prognostic value and immune infiltration of DSC2 in OS via single cell and bulk RNA seq data. Besides, the expression and function of DSC2 in OS were further verified by in vitro experiment. We preliminarily determined that DSC2 was high expressed in OS, which was a risk factor for survival and had a strong relationship with immune cell infiltration. What's more, in vitro experiments also demonstrated that DSC2 was high expressed in OS cells, and silencing DSC2 would suppress proliferation, migration and invasion of OS cells. DSC2 may serve as an oncogene, which exerts a crucial role in tumor progression, predicting prognosis and immune cell infiltration in OS.

摘要

骨肉瘤（OS）的病因尚不清楚。桥粒芯胶蛋白-2（DSC2）介导细胞间粘附并参与肿瘤进展。因此，我们旨在研究DSC2在骨肉瘤中的潜在作用。我们通过单细胞和批量RNA测序数据分析了DSC2在骨肉瘤中的表达、预后价值和免疫浸润情况。此外，通过体外实验进一步验证了DSC2在骨肉瘤中的表达和功能。我们初步确定DSC2在骨肉瘤中高表达，这是一个生存危险因素，并且与免疫细胞浸润密切相关。此外，体外实验还表明DSC2在骨肉瘤细胞中高表达，沉默DSC2会抑制骨肉瘤细胞的增殖、迁移和侵袭。DSC2可能作为一种癌基因，在骨肉瘤的肿瘤进展、预后预测和免疫细胞浸润中发挥关键作用。