聚酒石黄修饰铅笔石墨电极用于人血清和人工尿液中尿酸的电催化测定
Electrocatalytic Determination of Uric Acid with the Poly(Tartrazine)-Modified Pencil Graphite Electrode in Human Serum and Artificial Urine.
作者信息
Liv Lokman, Portakal Merve, Çukur Meryem Sıla, Topaçlı Beyza, Uzun Berkay
机构信息
Electrochemistry Laboratory, Chemistry Group, The Scientific and Technological Research Council of Turkey, National Metrology Institute, (TUBITAK UME), 41470 Gebze, Kocaeli, Turkey.
Faculty of Technology, Department of Biomedical Engineering, Pamukkale University, 20160 Denizli, Turkey.
出版信息
ACS Omega. 2023 Sep 11;8(38):34420-34430. doi: 10.1021/acsomega.3c02561. eCollection 2023 Sep 26.
A novel electrocatalytic sensing strategy was built for uric acid (UA) determination with an exceptionally developed poly(tartrazine)-modified activated pencil graphite electrode (pTRT/aPGE) in human serum and artificial urine. The oxidation signal of UA at 275 mV in pH 7.5 phosphate buffer solution served as the analytical response. Cyclic voltammetry, electrochemical impedance spectroscopy, scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray photoelectron spectroscopy were used to characterize the sensing platform, which was able to detect 0.10 μM of UA in the ranges of 0.34-60 and 70-140 μM. The samples of human serum and artificial urine were analyzed by both the pTRT/aPGE and the uricase-modified screen-printed electrode. The results were statistically evaluated and compared with each other within the confidence level of 95%, and no significant difference between the results was found.
构建了一种新型的电催化传感策略，用于在人血清和人工尿液中使用特别研制的聚酒石黄修饰的活性铅笔石墨电极（pTRT/aPGE）测定尿酸（UA）。在pH 7.5的磷酸盐缓冲溶液中，UA在275 mV处的氧化信号用作分析响应。采用循环伏安法、电化学阻抗谱、扫描电子显微镜、能量色散X射线光谱和X射线光电子能谱对传感平台进行表征，该平台能够在0.34 - 60 μM和70 - 140 μM范围内检测到0.10 μM的UA。用人血清和人工尿液样本分别通过pTRT/aPGE和尿酸酶修饰的丝网印刷电极进行分析。对结果进行了统计评估，并在95%的置信水平内相互比较，结果之间未发现显著差异。
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