Sarkar Subhabrata, Bora Ishani, Gupta Parakriti, Sapkal Gajanan, Shethi Shveta, Kaur Kanwalpreet, Ratho Radha Kanta
Department of Virology, Post Graduate Institute of Medical Education and Research, Chandigarh, National Institute of Virology, Pune, India.
Department of Medical Microbiology, Post Graduate Institute of Medical Education and Research, Chandigarh, National Institute of Virology, Pune, India.
Virusdisease. 2023 Sep;34(3):365-372. doi: 10.1007/s13337-023-00831-0. Epub 2023 Jul 26.
Nucleic acid amplification tests (NAATs) have revolutionized reliable detection of dengue virus (DENV) during acute phase of infection. The study evaluated performance of CDC DENV-1-4 real-time assay, trioplex RT-PCR and heminested conventional RT-PCR assay in the diagnosis of DENV. The three NAATs were performed on 107 consecutive samples collected from patients suspected of DENV infection during acute phase of illness. Their performance was compared against composite reference standard, consisting of DENV NS1 antigen ELISA and DENV IgM ELISA. 88/107 study samples were positive by DENV ELISA, either NS1Ag (80), IgM (3) or both (5). The overall sensitivity of CDC DENV-1-4 RT-PCR assay, trioplex RT-PCR assay and conventional multiplex RT-PCR was 68.18%, 54.55% and 38.64%, respectively in diagnosing dengue during acute phase, with an area under the curve of 0.841, 0.773 and 0.693 respectively when compared against composite reference standard. The sensitivity was 82.93%, 73.17% and 51.22%, respectively within three days of illness and 60%, 42.86% and 28.57%, respectively between 4 and 5th day of illness. All the three molecular assays had 100% specificity. Maximum concordance values of 86.9% were recorded among CDC DENV-1-4 rRT-PCR assay and trioplex assay with kappa value of 0.74, suggestive of substantial agreement. CDC DENV-1-4 rRT-PCR assay can be used as a reliable and accurate test for diagnosis of DENV during acute phase of illness.
核酸扩增检测（NAATs）彻底改变了在感染急性期对登革热病毒（DENV）的可靠检测。该研究评估了美国疾病控制与预防中心（CDC）DENV - 1 - 4实时检测法、三重RT - PCR和半巢式常规RT - PCR检测法在登革热病毒诊断中的性能。这三种NAATs对107份连续样本进行了检测，这些样本采自疾病急性期疑似感染登革热病毒的患者。将它们的性能与由DENV NS1抗原酶联免疫吸附测定（ELISA）和DENV IgM ELISA组成的综合参考标准进行了比较。107份研究样本中，有88份通过DENV ELISA呈阳性，其中NS1抗原阳性80份、IgM阳性3份或两者均阳性5份。在疾病急性期诊断登革热时，CDC DENV - 1 - 4 RT - PCR检测法、三重RT - PCR检测法和常规多重RT - PCR的总体敏感性分别为68.18%、54.55%和38.64%，与综合参考标准相比，曲线下面积分别为0.841、0.773和0.693。在发病三天内，敏感性分别为82.93%、73.17%和51.22%；在发病第4至5天，敏感性分别为60%、42.
