比较美国食品药品监督管理局（FDA）批准的疾病预防控制中心（CDC）登革热病毒 1-4 型实时逆转录聚合酶链反应（RT-PCR）与实验室开发的登革热病毒检测和血清分型检测方法。
Comparison of the FDA-approved CDC DENV-1-4 real-time reverse transcription-PCR with a laboratory-developed assay for dengue virus detection and serotyping.
机构信息
Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, California, USA.
出版信息
J Clin Microbiol. 2013 Oct;51(10):3418-20. doi: 10.1128/JCM.01359-13. Epub 2013 Jul 31.
Abstract
Dengue virus (DENV) is the agent of the most common vector-borne disease worldwide. Using 199 clinical samples collected from Nicaragua and Sri Lanka, a laboratory-developed DENV multiplex real-time reverse transcription-PCR (rRT-PCR) proved more clinically sensitive than the FDA-approved CDC assay for DENV serotypes 1 to 4 when measured against a composite reference standard, with sensitivities of 97.4% versus 87.1%, respectively.
摘要
登革热病毒（DENV）是全球最常见的虫媒病毒病的病原体。使用从尼加拉瓜和斯里兰卡收集的 199 份临床样本，实验室开发的 DENV 多重实时逆转录聚合酶链反应（rRT-PCR）与复合参考标准相比，对 DENV 血清型 1 至 4 的检测结果比美国食品和药物管理局（FDA）批准的疾病预防控制中心（CDC）检测方法更具临床敏感性，其敏感性分别为 97.4%和 87.1%。
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