Tula State University, pr. Lenina 92, 300012 Tula, Russia.
Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky pr. 47, 119991 Moscow, Russia.
ACS Appl Mater Interfaces. 2023 Oct 11;15(40):47779-47789. doi: 10.1021/acsami.3c09897. Epub 2023 Oct 2.
This study presents a novel ″3-in-1″ hybrid biocatalyst design that combines the individual efficiency of microorganisms while avoiding negative interactions between them. Yeast cells of VKM Y-2559, VKM Y-2677, and VKM Y-2482 were immobilized in an organosilicon material by using the sol-gel method, resulting in a hybrid biocatalyst. The catalytic activity of the immobilized microorganism mixture was evaluated by employing it as the bioreceptor element of a biosensor. Optical and scanning electron microscopies were used to examine the morphology of the biohybrid material. Elemental distribution analysis confirmed the encapsulation of yeast cells in a matrix composed of methyltriethoxysilane (MTES) and tetraethoxysilane (TEOS) (85 and 15 vol %, respectively). The resulting heterogeneous biocatalyst exhibited excellent performance in determining the biochemical oxygen demand (BOD) index in real surface water samples, with a sensitivity coefficient of 50 ± 3 × 10·min, a concentration range of 0.3-31 mg/L, long-term stability for 25 days, and a relative standard deviation of 3.8%. These findings demonstrate the potential of the developed hybrid biocatalyst for effective pollution monitoring and wastewater treatment applications.
本研究提出了一种新颖的“三合一”混合生物催化剂设计，它结合了微生物的个体效率，同时避免了它们之间的负面相互作用。使用溶胶-凝胶法将 VKM Y-2559、VKM Y-2677 和 VKM Y-2482 的酵母细胞固定在有机硅材料中，得到了一种混合生物催化剂。通过将固定化微生物混合物用作生物传感器的生物受体元件，评估了其催化活性。使用光学显微镜和扫描电子显微镜检查了生物杂化材料的形态。元素分布分析证实了酵母细胞被包裹在由甲基三乙氧基硅烷（MTES）和四乙氧基硅烷（TEOS）（分别为 85 和 15 体积％）组成的基质中。所得的非均相生物催化剂在测定实际地表水样品中的生化需氧量（BOD）指数方面表现出优异的性能，灵敏度系数为 50±3×10·min，浓度范围为 0.3-31mg/L，25 天的长期稳定性和相对标准偏差为 3.8%。这些发现表明，所开发的混合生物催化剂具有有效监测污染和处理废水的应用潜力。
