Tula State University, Pr. Lenina 92, Tula 300012, Russia.
Laboratory of Cytology of Microorganisms, Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center «Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences», Pr. Nauki 5, Pushchino, Moscow Region 142290, Russia.
Enzyme Microb Technol. 2021 Oct;150:109879. doi: 10.1016/j.enzmictec.2021.109879. Epub 2021 Jul 27.
The impact of hydrophilic polymers in an organosilica matrix on the features and performance of immobilized methylotrophic yeast cells used as biocatalysts was investigated and described. Yeast cells were immobilized in a matrix made of tetraethoxysilane (TEOS) and methyltriethoxysilane (MTES) by one-step sol-gel route of synthesis in the presence of polyethylene glycol (PEG) or polyvinyl alcohol (PVA). Organosilica shells were spontaneously built around cells as a result of yeast immobilization at a TEOS to MTES ratio of 85/15 vol% and hydrophilic polymer (PEG or PVA). As a structure-directing agent, PVA produces organosilica films. Stable high-performance biocatalysts active for one year, if stored at -18 °C, have been obtained by entrapment of methylotrophic yeast cells. A trickling biofilter with and without active aeration was designed using entrapped yeast cells to treat methanol polluted wastewater. A biofilter model with active aeration could halve methanol input thus demonstrating better performance compared to treatment without active aeration.
研究并描述了有机硅基质中亲水性聚合物对用作生物催化剂的固定化甲基营养酵母细胞的特性和性能的影响。酵母细胞通过一步溶胶-凝胶法在聚乙二醇（PEG）或聚乙烯醇（PVA）存在下固定在由四乙氧基硅烷（TEOS）和甲基三乙氧基硅烷（MTES）制成的基质中。由于在 TEOS 与 MTES 的体积比为 85/15 的情况下以及亲水性聚合物（PEG 或 PVA）存在下对酵母进行固定化，因此亲水性聚合物（PEG 或 PVA）会在细胞周围自发形成有机硅壳。作为结构导向剂，PVA 会产生有机硅薄膜。如果储存在-18°C 下，可以通过包埋甲基营养酵母细胞获得稳定的高性能生物催化剂，其活性可维持一年。使用包埋的酵母细胞设计了带有和不带有主动曝气的滴流生物过滤器，以处理甲醇污染的废水。与不进行主动曝气的处理相比，具有主动曝气的生物过滤器模型可以将甲醇输入减半，从而显示出更好的性能。
